Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance
| العنوان: | Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance |
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| المؤلفون: | Fang Zhan, Yu Chao Wang, Xue Peng Cai, Hong Bin Yan, Hao Tai Chen, Wan Gui Shi, Shaohua Zhang, Xing Quan Zhu, Wan Zhong Jia, D. Timothy J. Littlewood, Bao Quan Fu, Ai Jiang Guo |
| المصدر: | BMC Genomics ResearcherID BMC Genomics, Vol 11, Iss 1, p 447 (2010) |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | medicine.medical_specialty, Mitochondrial DNA, lcsh:QH426-470, lcsh:Biotechnology, Population genetics, Biology, Genome, Molecular ecology, RNA, Transfer, Genus, Phylogenetics, lcsh:TP248.13-248.65, Molecular genetics, parasitic diseases, Genetics, medicine, Animals, Humans, Phylogeny, Taenia, Genetic Variation, biology.organism_classification, lcsh:Genetics, Genome, Mitochondrial, Research Article, Biotechnology |
| الوصف: | Background Mitochondrial genomes provide a rich source of molecular variation of proven and widespread utility in molecular ecology, population genetics and evolutionary biology. The tapeworm genus Taenia includes a diversity of tapeworm parasites of significant human and veterinary importance. Here we add complete sequences of the mt genomes of T. multiceps, T. hydatigena and T. pisiformis, to a data set of 4 published mtDNAs in the same genus. Seven complete mt genomes of Taenia species are used to compare and contrast variation within and between genomes in the genus, to estimate a phylogeny for the genus, and to develop novel molecular markers as part of an extended mitochondrial toolkit. Results The complete circular mtDNAs of T. multiceps, T. hydatigena and T. pisiformis were 13,693, 13,492 and 13,387 bp in size respectively, comprising the usual complement of flatworm genes. Start and stop codons of protein coding genes included those found commonly amongst other platyhelminth mt genomes, but the much rarer initiation codon GTT was inferred for the gene atp 6 in T. pisiformis. Phylogenetic analysis of mtDNAs offered novel estimates of the interrelationships of Taenia. Sliding window analyses showed nad 6, nad 5, atp 6, nad 3 and nad 2 are amongst the most variable of genes per unit length, with the highest peaks in nucleotide diversity found in nad 5. New primer pairs capable of amplifying fragments of variable DNA in nad 1, rrn S and nad 5 genes were designed in silico and tested as possible alternatives to existing mitochondrial markers for Taenia. Conclusions With the availability of complete mtDNAs of 7 Taenia species, we have shown that analysis of amino acids provides a robust estimate of phylogeny for the genus that differs markedly from morphological estimates or those using partial genes; with implications for understanding the evolutionary radiation of important Taenia. Full alignment of the nucleotides of Taenia mtDNAs and sliding window analysis suggests numerous alternative gene regions are likely to capture greater nucleotide variation than those currently pursued as molecular markers. New PCR primers developed from a comparative mitogenomic analysis of Taenia species, extend the use of mitochondrial markers for molecular ecology, population genetics and diagnostics. |
| تدمد: | 1471-2164 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b5af6839ebbe6650402c4efca60dba3 https://doi.org/10.1186/1471-2164-11-447 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3b5af6839ebbe6650402c4efca60dba3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712164 |
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