The Positive Effect of MiR1 Antagomir on Ischemic Neurological Disorders Via Changing the Expression of Bcl-w and Bad Genes
| العنوان: | The Positive Effect of MiR1 Antagomir on Ischemic Neurological Disorders Via Changing the Expression of Bcl-w and Bad Genes |
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| المؤلفون: | Anis Talebi, Mohammad Reza Bigdeli, Mehdi Rahnema |
| المصدر: | Basic and Clinical Neuroscience, Vol 11, Iss 6, Pp 811-820 (2020) Basic and Clinical Neuroscience |
| بيانات النشر: | Negah Scientific Publisher, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Neurology, medicine.medical_treatment, Pharmacology, infarct volume, bcl-w, Neuroprotection, lcsh:RC321-571, 03 medical and health sciences, Cellular and Molecular Neuroscience, chemistry.chemical_compound, 0302 clinical medicine, microRNA, medicine, Antagomir, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Stroke, Saline, mir1, Clinical neuroscience, apoptosis, medicine.disease, stroke, antagomir, 030104 developmental biology, chemistry, Apoptosis, bad, Neurology (clinical), 030217 neurology & neurosurgery, Research Paper |
| الوصف: | Introduction: MicroRNAs (miRNAs or miRs) are non-coding RNAs. Studies have shown that miRNAs are expressed aberrantly in stroke. The miR1 enhances ischemic damage, and a previous study has demonstrated that reduction of miR1 level has a neuroprotective effect on the Middle Cerebral Artery Occlusion (MCAO). Since apoptosis is one of the important processes in neural protection, the possible effect of miR1 on this pathway has been tested in this study. Post-ischemic administration of miR1 antagomir reduces infarct volume via bcl-w and bad expression. Methods: Rats were divided into four experimental groups: sham, control, positive control, and antagomir treatment group. One hour after MCAO surgery, the rats were received intravenously (Tail vein) 0.1 mL Normal Saline (NS), 0.1 mL rapamycin, and 300 pmol/g miR1 antagomir (soluble in 0.1 mL normal saline) in control, positive control, and treatment group, respectively. Twenty-four hours after reperfusion infarct volume was measured. The expression of miR1, bcl-w, and bad were analyzed using real-time PCR in sham, control, and treated groups. Results: Our results indicate that administration of miR1 antagomir reduces infarct volume significantly, it also decreases miR1 and bad expression while increases bcl-w expression. Conclusion: Understanding the precise neuroprotective mechanism of miR1 antagomir can make it a proper treatment and an innovative approach for stroke therapy. |
| تدمد: | 2008-126X 2228-7442 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bd35cdb01ee911596d74acf21941deb https://doi.org/10.32598/bcn.11.6.324.3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3bd35cdb01ee911596d74acf21941deb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2008126X 22287442 |
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