Estrogen receptor profiles across tissues from male and female Rattus norvegicus
| العنوان: | Estrogen receptor profiles across tissues from male and female Rattus norvegicus |
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| المؤلفون: | Benard O. Ogola, Sarah H. Lindsey, Ricardo Mostany, Rakesh Gurrala, Margaret A. Zimmerman, Ryousuke Satou, Dillion D. Hutson |
| المصدر: | Biology of Sex Differences Biology of Sex Differences, Vol 10, Iss 1, Pp 1-13 (2019) |
| بيانات النشر: | BioMed Central, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, medicine.drug_class, Estrogen receptor, lcsh:Medicine, Estrogen receptors, Cardiovascular, lcsh:Physiology, Cell Line, Gender Studies, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Aromatase, Internal medicine, Gene expression, Sex differences, medicine, Animals, RNA, Messenger, Receptor, Messenger RNA, Sex Characteristics, biology, lcsh:QP1-981, Research, lcsh:R, 030104 developmental biology, Receptors, Estrogen, Estrogen, biology.protein, Female, Droplet digital PCR (ddPCR), Transcriptome, GPER, Estrogen receptor alpha, 030217 neurology & neurosurgery |
| الوصف: | Background Estrogen is formed by the enzyme aromatase (CYP19A1) and signals via three identified receptors ERα (ESR1), ERß (ESR2), and the G protein-coupled estrogen receptor (GPER). Understanding the relative contribution of each receptor to estrogenic signaling may elucidate the disparate effects of this sex hormone across tissues, and recent developments in PCR technology allow absolute quantification and direct comparison of multiple targets. We hypothesized that this approach would reveal tissue- and sex-specific differences in estrogen receptor mRNA. Methods ESR1, ESR2, GPER, and CYP19A1 were measured in four cardiovascular tissues (heart, aorta, kidney, and adrenal gland), three brain areas (somatosensory cortex, hippocampus, and prefrontal cortex), and reproductive tissues (ovaries, mammary gland, uterus, testes) from six male and six female adult Sprague-Dawley rats. Results GPER mRNA expression was relatively stable across all tissues in both sexes, ranging from 5.49 to 113 copies/ng RNA, a 21-fold difference. In contrast, ESR1/ESR2 were variable across tissues although similar within an organ system. ESR1 ranged from 4.46 to 614 copies/ng RNA (138-fold difference) while ESR2 ranged from 0.154 to 83.1 copies/ng RNA (540-fold). Significant sex differences were broadly absent except for renal ESR1 (female 206 vs. male 614 copies/ng RNA, P |
| اللغة: | English |
| تدمد: | 2042-6410 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c35dff0986281f86503503cc8167f6d http://europepmc.org/articles/PMC6329134 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3c35dff0986281f86503503cc8167f6d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20426410 |
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