Immuno-modulating properties of Tulathromycin in porcine monocyte-derived macrophages infected with porcine reproductive and respiratory syndrome virus
| العنوان: | Immuno-modulating properties of Tulathromycin in porcine monocyte-derived macrophages infected with porcine reproductive and respiratory syndrome virus |
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| المؤلفون: | Affan Siddiq, Robin M. Yates, Gregory Muench, Andre G. Buret, Troy D Feener, D. Desmonts de Lamache, Thibault Allain, Ruth Moges, Neil McKenna |
| المصدر: | PLOS ONE PLoS ONE, Vol 14, Iss 8, p e0221560 (2019) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Pulmonology, Swine, Sialic Acid Binding Ig-like Lectin 1, animal diseases, viruses, Intracellular Space, Apoptosis, Pathology and Laboratory Medicine, Disaccharides, Virus Replication, Monocytes, Pathogenesis, White Blood Cells, Mice, Animal Cells, Heterocyclic Compounds, Medicine and Health Sciences, Macrophage, Tulathromycin, Mammals, Multidisciplinary, biology, Cell Death, Eukaryota, virus diseases, Cell Differentiation, General Medicine, Opsonin Proteins, Interleukin-10, Mitochondria, Cell Processes, Vertebrates, Receptors, Virus, Medicine, Female, medicine.symptom, Cellular Types, General Agricultural and Biological Sciences, Research Article, Immune Cells, Science, Immunology, Porcine Reproductive and Respiratory Syndrome, Antigens, Differentiation, Myelomonocytic, Inflammation, Context (language use), Receptors, Cell Surface, Virus, General Biochemistry, Genetics and Molecular Biology, Microbiology, Cell Line, Necrosis, Signs and Symptoms, Phagocytosis, Diagnostic Medicine, Antigens, CD, medicine, Animals, Immunologic Factors, Porcine respiratory and reproductive syndrome virus, Cell Shape, Blood Cells, Macrophages, Interleukin-8, Organisms, Biology and Life Sciences, Cell Biology, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Respiratory Infections, Amniotes, Reactive Oxygen Species, CD163, Developmental Biology |
| الوصف: | Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-stranded RNA virus that grows in macrophages and causes acute pneumonia in pigs. PRRSV causes devastating losses to the porcine industry. However, due to its high antigenic variability and poorly understood immunopathogenesis, there is currently no effective vaccine or treatment to control PRRSV infection. The common occurrence of PRRSV infection with bacterial infections as well as its inflammatory-driven pathobiology raises the question of the value of antibiotics with immunomodulating properties for the treatment of the disease it causes. The macrolide antibiotic Tulathromycin (TUL) has been found to exhibit potent anti-inflammatory and immunomodulating properties in cattle and pigs. The aim of this study was to characterize the anti-viral and immunomodulating properties of TUL in PRRSV-infected porcine macrophages. Our findings indicate that blood monocyte-derived macrophages are readily infected by PRRSV and can be used as an effective cellular model to study PRRSV pathogenesis. TUL did not change intracellular or extracellular viral titers, not did it alter viral receptors (CD163 and CD169) expression on porcine macrophages. In contrast, TUL exhibited potent immunomodulating properties, which therefore occurred in the absence of any direct antiviral effects against PRRSV. TUL had an additive effect with PRRSV on the induction of macrophage apoptosis, and inhibited virus-induced necrosis. TUL significantly attenuated PRRSV-induced macrophage pro-inflammatory signaling (CXCL-8 and mitochondrial ROS production) and prevented PRRSV inhibition of non-opsonized and opsonized phagocytic function. Together, these data demonstrate that TUL inhibits PRRSV-induced inflammatory responses in porcine macrophages and protects against the phagocytic impairment caused by the virus. Research in live pigs is warranted to assess the potential clinical benefits of this antibiotic in the context of virally induced inflammation and tissue injury. |
| تدمد: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0221560 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c7d4d12fef7d1961de4ca536954dcfa |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3c7d4d12fef7d1961de4ca536954dcfa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0221560 |