Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin
| العنوان: | Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin |
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| المؤلفون: | Mimoko Santo, Kyoko Suzuki-Takedachi, Shigemasa Hanazawa, Toshiaki Kurogi, Yoshihiro Takihara, Yoshinori Ohno, Yoshikazu Masuhiro, Motoaki Ohtsubo, Kazuhito Naka, Shin'ichiro Yasunaga |
| المصدر: | PLoS ONE PLoS ONE, Vol 11, Iss 5, p e0155558 (2016) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription, Genetic, Hydrolases, Amino Acid Motifs, lcsh:Medicine, Gene Expression, Synthesis Phase, Cell Cycle Proteins, Biochemistry, DNA replication factor CDT1, Mice, Cell Cycle and Cell Division, lcsh:Science, Multidisciplinary, biology, Chromosome Biology, Cell Cycle, Nuclear Proteins, Cell cycle, Chromatin, Recombinant Proteins, Cell biology, Enzymes, DNA-Binding Proteins, Cell Processes, embryonic structures, Cell lines, Epigenetics, Biological cultures, Plasmids, Research Article, DNA Replication, Proteasome Endopeptidase Complex, Nucleases, DNA transcription, Fibroblast Growth Factor 4, Real-Time Polymerase Chain Reaction, Transfection, Chromatin remodeling, 03 medical and health sciences, NIH 3T3 cells, Genetics, Animals, Humans, Gene Regulation, Cell Cycle Protein, Transcription factor, Ubiquitin, lcsh:R, DNA Helicases, Geminin, Biology and Life Sciences, Proteins, Cell Biology, Fibroblasts, Chromatin Assembly and Disassembly, Mice, Inbred C57BL, Research and analysis methods, 030104 developmental biology, RAW 264.7 Cells, Gene Expression Regulation, Cancer cell, biology.protein, Enzymology, lcsh:Q, K562 Cells, Transcription Factors |
| الوصف: | Geminin regulates chromatin remodeling and DNA replication licensing which play an important role in regulating cellular proliferation and differentiation. Transcription of the Geminin gene is regulated via an E2F-responsive region, while the protein is being closely regulated by the ubiquitin-proteasome system. Our objective was to directly transduce Geminin protein into cells. Recombinant cell-penetrating Geminin (CP-Geminin) was generated by fusing Geminin with a membrane translocating motif from FGF4 and was efficiently incorporated into NIH 3T3 cells and mouse embryonic fibroblasts. The withdrawal study indicated that incorporated CP-Geminin was quickly reduced after removal from medium. We confirmed CP-Geminin was imported into the nucleus after incorporation and also that the incorporated CP-Geminin directly interacted with Cdt1 or Brahma/Brg1 as the same manner as Geminin. We further demonstrated that incorporated CP-Geminin suppressed S-phase progression of the cell cycle and reduced nuclease accessibility in the chromatin, probably through suppression of chromatin remodeling, indicating that CP-Geminin constitutes a novel tool for controlling chromatin configuration and the cell cycle. Since Geminin has been shown to be involved in regulation of stem cells and cancer cells, CP-Geminin is expected to be useful for elucidating the role of Geminin in stem cells and cancer cells, and for manipulating their activity. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c855b223fb5eba81ddf3ed53711c4d6 https://pubmed.ncbi.nlm.nih.gov/27195810 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3c855b223fb5eba81ddf3ed53711c4d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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