Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish
| العنوان: | Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish |
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| المؤلفون: | Hugo Poplimont, Julian Patt, Nicole Merten, Antonios Georgantzoglou, Elisabeth M. Busch-Nentwich, Christina Kotsi, Caroline Coombs, Evi Kostenis, Milka Sarris, Sarah Williams, Hazel A. Walker |
| المساهمون: | Georgantzoglou, Antonios [0000-0003-4418-8749], Patt, Julian [0000-0001-5736-0283], Merten, Nicole [0000-0002-8487-6805], Poplimont, Hugo [0000-0002-2319-363X], Busch-Nentwich, Elisabeth M [0000-0001-6450-744X], Kostenis, Evi [0000-0001-8284-5514], Sarris, Milka [0000-0001-7016-333X], Apollo - University of Cambridge Repository, Busch-Nentwich, Elisabeth M. [0000-0001-6450-744X] |
| المصدر: | Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019) Nature Communications |
| بيانات النشر: | Apollo - University of Cambridge Repository, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Time Factors, Neutrophils, 96, General Physics and Astronomy, 13, 14, 96/31, 59, Receptors, Interleukin-8B, Receptors, Interleukin-8A, Chemokine receptor, 0302 clinical medicine, Cell Movement, CXC chemokine receptors, 14/19, Receptor, lcsh:Science, Zebrafish, 64, Multidisciplinary, biology, Chemotaxis, 631/80/84/1372, article, 96/21, Endocytosis, Cell biology, Protein Transport, Imaging the immune system, 631/80/84/2337, Science, 631/250/2503, Motility, Down-Regulation, 13/109, 96/10, Models, Biological, 631/250/2504/223/1699, General Biochemistry, Genetics and Molecular Biology, 38, 96/95, 03 medical and health sciences, 13/21, Downregulation and upregulation, Live cell imaging, 38/88, Animals, Amino Acid Sequence, 14/35, 64/116, Cell Membrane, General Chemistry, Zebrafish Proteins, biology.organism_classification, 030104 developmental biology, Mutagenesis, 14/63, Mutation, Amoeboid migration, Wounds and Injuries, lcsh:Q, 030217 neurology & neurosurgery |
| الوصف: | Immune cells congregate at specific loci to fight infections during inflammatory responses, a process that must be transient and self-resolving. Cell dispersal promotes resolution, but it remains unclear how transition from clustering to dispersal is regulated. Here we show, using quantitative live imaging in zebrafish, that differential ligand-induced trafficking of chemokine receptors such as Cxcr1 and Cxcr2 orchestrates the state of neutrophil congregation at sites of tissue damage. Through receptor mutagenesis and biosensors, we show that Cxcr1 promotes clustering at wound sites, but is promptly desensitized and internalized, which prevents excess congregation. By contrast, Cxcr2 promotes bidirectional motility and is sustained at the plasma membrane. Persistent plasma membrane residence of Cxcr2 prolongs downstream signaling and is required for sustained exploratory motion conducive to dispersal. Thus, differential trafficking of two chemokine receptors allows coordination of antagonistic cell behaviors, promoting a self-resolving migratory response. Inflammatory responses must be induced and resolved timely to serve protection from pathogens without inducing excessive tissue damage. Here the authors use live imaging in zebrafish to show that the intracellular trafficking of two chemokine receptors, Cxcr1 and Cxcr2, is differentially regulated on activated neutrophils to control their clustering and dispersal, respectively. |
| وصف الملف: | application/pdf; application/zip; text/xml |
| DOI: | 10.17863/cam.47075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d3f540bbc72986aefb29241ae01fa20 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3d3f540bbc72986aefb29241ae01fa20 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.17863/cam.47075 |
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