The Inflammation Biomarker GlycA Reflects Plasma N-Glycan Branching
| العنوان: | The Inflammation Biomarker GlycA Reflects Plasma N-Glycan Branching |
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| المؤلفون: | Maxence Noel, Daniel I Chasman, Samia Mora, James D Otvos, Christopher D Palmer, Patrick J Parsons, Jordan W Smoller, Richard D Cummings, Robert G Mealer |
| المصدر: | Clinical Chemistry. 69:80-87 |
| بيانات النشر: | Oxford University Press (OUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biochemistry (medical), Clinical Biochemistry |
| الوصف: | Background GlycA is a nuclear magnetic resonance (NMR) signal in plasma that correlates with inflammation and cardiovascular outcomes in large data sets. The signal is thought to originate from N-acetylglucosamine (GlcNAc) residues of branched plasma N-glycans, though direct experimental evidence is limited. Trace element concentrations affect plasma glycosylation patterns and may thereby also influence GlycA. Methods NMR GlycA signal was measured in plasma samples from 87 individuals and correlated with MALDI-MS N-glycomics and trace element analysis. We further evaluated the genetic association with GlycA at rs13107325, a single nucleotide polymorphism resulting in a missense variant within SLC39A8, a manganese transporter that influences N-glycan branching, both in our samples and existing genome-wide association studies data from 22 835 participants in the Women’s Health Study (WHS). Results GlycA signal was correlated with both N-glycan branching (r2 ranging from 0.125–0.265; all P < 0.001) and copper concentration (r2 = 0.348, P < 0.0001). In addition, GlycA levels were associated with rs13107325 genotype in the WHS (β [standard error of the mean] = −4.66 [1.2674], P = 0.0002). Conclusions These results provide the first direct experimental evidence linking the GlycA NMR signal to N-glycan branching commonly associated with acute phase reactive proteins involved in inflammation. |
| تدمد: | 1530-8561 0009-9147 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d67a43c44b3ba11505b16812bdd6dae https://doi.org/10.1093/clinchem/hvac160 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....3d67a43c44b3ba11505b16812bdd6dae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15308561 00099147 |
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