Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs
| العنوان: | Thermotoga profunda sp. nov. and Thermotoga caldifontis sp. nov., anaerobic thermophilic bacteria isolated from terrestrial hot springs |
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| المؤلفون: | Ken-ichiro Suzuki, Akira Hosoyama, Nobuyuki Fujita, Koji Mori, Shoko Ohji, Hiroyuki Kimura, Atsushi Yamazoe, Jun-ichiro Ishibashi |
| المصدر: | International Journal of Systematic and Evolutionary Microbiology. 64:2128-2136 |
| بيانات النشر: | Microbiology Society, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | DNA, Bacterial, Sequence analysis, Molecular Sequence Data, Biology, Microbiology, Hot Springs, Gram-Negative Anaerobic Straight, Curved, and Helical Rods, Japan, Ribosomal protein, RNA, Ribosomal, 16S, Thermotoga hypogea, education, Phylogeny, Ecology, Evolution, Behavior and Systematics, Base Composition, education.field_of_study, Strain (chemistry), Thermophile, Fatty Acids, Nucleic Acid Hybridization, Sequence Analysis, DNA, General Medicine, Ribosomal RNA, 16S ribosomal RNA, biology.organism_classification, Bacterial Typing Techniques, Bacteria |
| الوصف: | Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06T and AZM44c09T, were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06T were 60 °C, pH 7.4 and 0 % additional NaCl, and those for strain AZM44c09T were 70 °C, pH 7.4 and 0 % additional NaCl. Complete genome sequencing was performed for both strains, revealing genome sizes of 2.19 Mbp (AZM34c06T) and 2.01 Mbp (AZM44c09T). Phylogenetic analyses based on 16S rRNA gene sequences and the concatenated predicted amino acid sequences of 33 ribosomal proteins showed that both strains belonged to the genus Thermotoga . The closest relatives of strains AZM34c06T and AZM44c09T were the type strains of Thermotoga lettingae (96.0 % similarity based on the 16S rRNA gene and 84.1 % similarity based on ribosomal proteins) and Thermotoga hypogea (98.6 and 92.7 % similarity), respectively. Using blast, the average nucleotide identity was 70.4–70.5 % when comparing strain AZM34c06T and T. lettingae TMOT and 76.6 % when comparing strain AZM44c09T and T. hypogea NBRC 106472T. Both values are far below the 95 % threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Thermotoga within two novel species, Thermotoga profunda sp. nov. (type strain AZM34c06T = NBRC 106115T = DSM 23275T) and Thermotoga caldifontis sp. nov. (type strain AZM44c09T = NBRC 106116T = DSM 23272T). |
| تدمد: | 1466-5034 1466-5026 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dae3bd3eeb815864cc770c34d8ab35a https://doi.org/10.1099/ijs.0.060137-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3dae3bd3eeb815864cc770c34d8ab35a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14665034 14665026 |
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