Analysis of Human Immunodeficiency Virus Type 1 Integration by Using A Specific, Sensitive and Quantitative Assay Based on Real-time Polymerase Chain Reaction
| العنوان: | Analysis of Human Immunodeficiency Virus Type 1 Integration by Using A Specific, Sensitive and Quantitative Assay Based on Real-time Polymerase Chain Reaction |
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| المؤلفون: | Yu Feng Wu, Hitoshi Ogasawara, Myint Oo Chang, Chika Tanaka, Toshio Naito, Yoshio Inagaki, Iwao Sekigawa, Yasunori Saito, Norio Yamamoto, Yasuo Hayashida |
| المصدر: | Virus Genes. 32:105-113 |
| بيانات النشر: | Springer Science and Business Media LLC, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Virus Integration, Integrase inhibitor, In Vitro Techniques, Biology, Polymerase Chain Reaction, Sensitivity and Specificity, Jurkat cells, Cell Line, law.invention, Jurkat Cells, chemistry.chemical_compound, law, Virology, Genetics, Humans, DNA Integration, Molecular Biology, Polymerase chain reaction, HIV Long Terminal Repeat, Base Sequence, U937 Cells, General Medicine, Molecular biology, Kinetics, Real-time polymerase chain reaction, chemistry, DNA, Viral, HIV-1, Leukocytes, Mononuclear, Nested polymerase chain reaction, DNA |
| الوصف: | A novel real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type-1 (HIV-1) DNA with high specificity and sensitivity. This assay reproducibly allowed the detection of three copies of integrated HIV DNA in a background of 100,000 cell equivalents of human chromosomal DNA. The non-specific amplification of unintegrated HIV-1 DNA was significantly inhibited in this assay and the specificity of this assay was much higher than the previously reported method. This assay showed that kinetics in viral DNA synthesis was cell-type dependent and that the kinetics of HIV-1 DNA integration was very rapid in Jurkat T cell line. This method may provide new insights into the integration processes and be useful in evaluating future integrase inhibitors. |
| تدمد: | 1572-994X 0920-8569 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dc1885ec6f56cbeb545aa3de67e31cd https://doi.org/10.1007/s11262-005-5851-2 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....3dc1885ec6f56cbeb545aa3de67e31cd |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1572994X 09208569 |
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