التفاصيل البيبلوغرافية
| العنوان: |
Leukocyte Isolation from Brain, Spinal Cord, and Meninges for Flow Cytometric Analysis |
| المؤلفون: |
Monica Manglani, Dorian B. McGavern, Selamawit Gossa |
| المصدر: |
Current protocols in immunology. 121(1) |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
0301 basic medicine, Immunology, Central nervous system, Cell Separation, Biology, Article, Flow cytometry, 03 medical and health sciences, Mice, Immune system, Meninges, medicine, Leukocytes, Animals, Autoimmune disease, medicine.diagnostic_test, Neurodegeneration, Brain, Spinal cord, medicine.disease, Flow Cytometry, 030104 developmental biology, medicine.anatomical_structure, Spinal Cord, Neuroscience, Homeostasis |
| الوصف: |
A continual dialogue exists between the central nervous system (CNS) and immune system that contributes to neural homeostasis as well as protection from microbes, repair following damage, autoimmune disease, and neurodegeneration. Characterization of resident and peripherally derived leukocyte populations within the central nervous system can provide valuable information regarding how these cells contribute to steady-state and inflammatory conditions. Flow cytometry provides a method to conduct detailed multi-parameter analyses of immune cells isolated from various tissues. This protocol provides a method to isolate leukocytes from brain, spinal cord, and meninges for flow cytometric analysis and provides a basic framework for phenotyping these cells. © 2018 by John Wiley & Sons, Inc. |
| تدمد: |
1934-368X |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e7d8b5ab89106ba7b033b4ae09553ee
https://pubmed.ncbi.nlm.nih.gov/30040211 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....3e7d8b5ab89106ba7b033b4ae09553ee |
| قاعدة البيانات: |
OpenAIRE |
