A Novel Europium Chelate Coated Nanosphere for Time-Resolved Fluorescence Immunoassay
| العنوان: | A Novel Europium Chelate Coated Nanosphere for Time-Resolved Fluorescence Immunoassay |
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| المؤلفون: | Donghua He, Yifeng Shen, Shaohan Xu |
| المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 6, p e0129689 (2015) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Lanthanide, Time Factors, Science, Fluoroimmunoassay, chemistry.chemical_element, Antibodies, chemistry.chemical_compound, Europium, Spectrophotometry, medicine, Chelation, Methylene, Edetic Acid, Chelating Agents, Multidisciplinary, Hepatitis B Surface Antigens, medicine.diagnostic_test, Staining and Labeling, Silicon Dioxide, Fluorescence, chemistry, Covalent bond, Reagent, Medicine, Spectrophotometry, Ultraviolet, Nanospheres, Nuclear chemistry, Research Article |
| الوصف: | A novel europium ligand 2, 2’, 2’’, 2’’’-(4, 7-diphenyl-1, 10-phenanthroline-2, 9-diyl) bis (methylene) bis (azanetriyl) tetra acetic acid (BC-EDTA) was synthesized and characterized. It shows an emission spectrum peak at 610 nm when it is excited at 360 nm, with a large Stock shift (250 nm). It is covalently coated on the surface of a bare silica nanosphere containi free amino groups, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-Hydroxysuccinimide. We also observed an interesting phenomenon that when BC-EDTA is labeled with a silica nanosphere, the chelate shows different excitation spectrum peaks of about 295 nm. We speculate that the carboxyl has a significant influence on its excitation spectrum. The BC-EDTA/Eu3+coated nanosphere could be used as a fluorescent probe for time-resolved fluorescence immunoassay. We labeled the antibody with the fluorescent nanosphere to develop a nanosphere based hepatitis B surface antigen as a time-resolved fluorescence immunoassay reagent, which is very easy to operate and eliminates potential contamination of Eu3+ contained in the environment. The analytical and functional sensitivities are 0.0037 μg/L and 0.08 μg/L (S/N≥2.0) respectively. The detection range is 0.08-166.67 μg/L, which is much wider than that of ELISA (0.2-5μg/L). It is comparable to the commercial dissociation-enhanced lanthanide fluoro-immunoassay system (DELFIA) reagents (0.2-145μg/L). We propose that it can fulfill clinical applications. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e8fc6931bfaa99f53deab3c23b261b1 https://pubmed.ncbi.nlm.nih.gov/26056826 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3e8fc6931bfaa99f53deab3c23b261b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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