Paraoxonase-2 Modulates Stress Response of Endothelial Cells to Oxidized Phospholipids and a Bacterial Quorum–Sensing Molecule
| العنوان: | Paraoxonase-2 Modulates Stress Response of Endothelial Cells to Oxidized Phospholipids and a Bacterial Quorum–Sensing Molecule |
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| المؤلفون: | Victor Grijalva, Zachary Port, Srinivasa T. Reddy, Sangderk Lee, Noam Bourquard, Yu-Rong Xia, Juyong Brian Kim, Casey E. Romanoski, Aldons J. Lusis, Diana M. Shih, Ke Wei Gong, Yi-Shou Shi, Yonghong Meng, Judith A. Berliner |
| المصدر: | Arteriosclerosis, Thrombosis, and Vascular Biology. 31:2624-2633 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Apoptosis, Inflammation, Biology, medicine.disease_cause, Article, Fight-or-flight response, 4-Butyrolactone, Stress, Physiological, Homoserine, medicine, Humans, RNA, Small Interfering, Aorta, Cells, Cultured, Phospholipids, Aryldialkylphosphatase, Pseudomonas aeruginosa, Quorum Sensing, food and beverages, biology.organism_classification, Cell biology, Lipoproteins, LDL, Quorum sensing, Chronic infection, Gene Expression Regulation, Unfolded protein response, Endothelium, Vascular, medicine.symptom, Cardiology and Cardiovascular Medicine, Oxidation-Reduction, Oxidative stress, Bacteria |
| الوصف: | Objective— Chronic infection has long been postulated as a stimulus for atherogenesis. Pseudomonas aeruginosa infection has been associated with increased atherosclerosis in rats, and these bacteria produce a quorum-sensing molecule 3-oxo-dodecynoyl-homoserine lactone (3OC12-HSL) that is critical for colonization and virulence. Paraoxonase 2 (PON2) hydrolyzes 3OC12-HSL and also protects against the effects of oxidized phospholipids thought to contribute to atherosclerosis. We now report the response of human aortic endothelial cells (HAECs) to 3OC12-HSL and oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (Ox-PAPC) in relation to PON2 expression. Methods and Results— Using expression profiling and network modeling, we identified the unfolded protein response (UPR), cell cycle genes, and the mitogen-activated protein kinase signaling pathway to be heavily involved in the HAEC response to 3OC12-HSL. The network also showed striking similarities to a network created based on HAEC response to Ox-PAPC, a major component of minimally modified low-density lipoprotein. HAECs in which PON2 was silenced by small interfering RNA showed increased proinflammatory response and UPR when treated with 3OC12-HSL or Ox-PAPC. Conclusion— 3OC12-HSL and Ox-PAPC influence similar inflammatory and UPR pathways. Quorum sensing molecules, such as 3OC12-HSL, contribute to the proatherogenic effects of chronic infection. The antiatherogenic effects of PON2 include destruction of quorum sensing molecules. |
| تدمد: | 1524-4636 1079-5642 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e97ea06b4f2164ff6319bfba0493610 https://doi.org/10.1161/atvbaha.111.232827 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3e97ea06b4f2164ff6319bfba0493610 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244636 10795642 |
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