Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID)
| العنوان: | Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID) |
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| المؤلفون: | Robert G. Roeder, Eric Sinn, Ananda L. Roy, Josephine Wang, Alexander Hoffmann, Masami Horikoshi, Tohru Yamamoto |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | Springer Science and Business Media LLC, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Genetics, Multidisciplinary, Base Sequence, TATA box, Molecular Sequence Data, Fungi, Computational biology, Plants, Biology, Biological Evolution, Conserved sequence, TAF1, TAF4, Sequence Homology, Nucleic Acid, Transcription Factor TFIID, Transcription preinitiation complex, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Transcription factor II D, Promoter Regions, Genetic, Transcription factor, Transcription Factors |
| الوصف: | The factor TFIID is one of several general factors that are necessary and sufficient for transcription initiation by mammalian RNA polymerase II. Stable interactions with the common TATA element lead both to template commitment and to the assembly of the other general factors into a functional preinitiation complex. Consistent with its key role in the promoter activation pathway, human TFIID also seems to be a target for some regulatory factors, as evidenced both by physical and functional studies of interactions between these components. The evolutionary conservation of functional properties led to the purification and cloning of yeast TFIID, the identification of presumptive structural motifs, and direct structure-function studies. Here we report the cloning of a complementary DNA encoding a functional human TFIID. This reveals an evolutionarily conserved core which corresponds precisely to the 180-residue DNA binding/activation domain determined for yeast TFIID, a near absolute conservation of component structural motifs (direct repeats, central basic core/lysine repeat, and sigma homology), providing further support for their functional importance, and a unique N-terminal structure that suggests involvement in species-specific regulatory factor interactions. |
| تدمد: | 1476-4687 0028-0836 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ea6b899096c66ca556c7d2d3cdaa66d https://doi.org/10.1038/346387a0 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....3ea6b899096c66ca556c7d2d3cdaa66d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 |
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