Vav2 is required for Netrin‐1 receptor‐class‐specific spinal motor axon guidance
| العنوان: | Vav2 is required for Netrin‐1 receptor‐class‐specific spinal motor axon guidance |
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| المؤلفون: | Wan Ling Tsai, Jian Ying Chuang, Yi Hsin Wu, Chih-Yang Wang, Meng Ting Wu, Tsung I. Hsu, Yi Syue Tsou, Tzu Jen Kao, Ming Yuan Chang |
| المصدر: | Developmental Dynamics. 251:444-458 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Motor Neurons, Nervous system, Neurite, Growth Cones, Netrin-1, Motor neuron, Biology, Axons, Axon Guidance, medicine.anatomical_structure, nervous system, Netrin, medicine, Biological neural network, Animals, Axon guidance, Axon, Proto-Oncogene Proteins c-vav, Growth cone, Neuroscience, Developmental Biology |
| الوصف: | BACKGROUND Proper guidance of neuronal axons to their targets is required to assemble neural circuits during the development of the nervous system. However, the mechanism by which the guidance of axonal growth cones is regulated by specific intermediaries activated by receptor signaling pathways to mediate cytoskeleton dynamics is unclear. Vav protein members have been proposed to mediate this process, prompting us to investigate their role in the limb selection of the axon trajectory of spinal lateral motor column (LMC) neurons. RESULTS We found Vav2 and Vav3 expression in LMC neurons when motor axons grew into the limb. Vav2, but not Vav3, loss-of-function perturbed LMC pathfinding, while Vav2 gain-of-function exhibited the opposite effects, demonstrating that Vav2 plays an important role in motor axon growth. Vav2 knockdown also attenuated the redirectional phenotype of LMC axons induced by Dcc, but not EphA4, in vivo and lateral LMC neurite growth preference to Netrin-1 in vitro. This study showed that Vav2 knockdown and ectopic nonphosphorylable Vav2 mutant expression abolished the Src-induced stronger growth preference of lateral LMC neurites to Netrin-1, suggesting that Vav2 is downstream of Src in this context. CONCLUSIONS Vav2 is essential for Netrin-1-regulated LMC motor axon pathfinding through Src interaction. |
| تدمد: | 1097-0177 1058-8388 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ee86cb19a120ddafaae8f198c1da2b8 https://doi.org/10.1002/dvdy.409 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....3ee86cb19a120ddafaae8f198c1da2b8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970177 10588388 |
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