Protein Tyrosine Phosphatase δ Mediates the Sema3A-Induced Cortical Basal Dendritic Arborization through the Activation of Fyn Tyrosine Kinase
| العنوان: | Protein Tyrosine Phosphatase δ Mediates the Sema3A-Induced Cortical Basal Dendritic Arborization through the Activation of Fyn Tyrosine Kinase |
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| المؤلفون: | Toshio Ohshima, Maria Shishikura, Stephen M. Strittmatter, Takeshi Yagi, Fumio Nakamura, Masahiko Taniguchi, Yoshio Goshima, Takako Okada, Noriko Uetani, Yoichiro Iwakura |
| المصدر: | The Journal of Neuroscience. 37:7125-7139 |
| بيانات النشر: | Society for Neuroscience, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Mice, Transgenic, Protein tyrosine phosphatase, Proto-Oncogene Proteins c-fyn, Gene Expression Regulation, Enzymologic, Mice, 03 medical and health sciences, FYN, Semaphorin, Animals, Research Articles, Cells, Cultured, Caenorhabditis elegans, Cerebral Cortex, Mice, Knockout, Neuronal Plasticity, biology, Kinase, General Neuroscience, Receptor-Like Protein Tyrosine Phosphatases, Class 2, Semaphorin-3A, SEMA3A, Dendrites, Protein-Tyrosine Kinases, biology.organism_classification, Cell biology, Enzyme Activation, Mice, Inbred C57BL, 030104 developmental biology, Female, Axon guidance, Neuroscience, Proto-oncogene tyrosine-protein kinase Src |
| الوصف: | Leukocyte common antigen-related (LAR) class protein tyrosine phosphatases (PTPs) are critical for axonal guidance; however, their relation to specific guidance cues is poorly defined. We here show that PTP-3, a LAR homolog in Caenorhabditis elegans, is involved in axon guidance regulated by Semaphorin-2A-signaling. PTPδ, one of the vertebrate LAR class PTPs, participates in the Semaphorin-3A (Sema3A)-induced growth cone collapse response of primary cultured dorsal root ganglion neurons from Mus musculus embryos. In vivo, however, the contribution of PTPδ in Sema3A-regualted axon guidance was minimal. Instead, PTPδ played a major role in Sema3A-dependent cortical dendritic growth. Ptpδ(−/−) and Sema3a(−/−) mutant mice exhibited poor arborization of basal dendrites of cortical layer V neurons. This phenotype was observed in both male and female mutants. The double-heterozygous mutants, Ptpδ(+/−); Sema3a(+/−), also showed a similar phenotype, indicating the genetic interaction. In Ptpδ(−/−) brains, Fyn and Src kinases were hyperphosphorylated at their C-terminal Tyr527 residues. Sema3A-stimulation induced dephosphorylation of Tyr527 in the dendrites of wild-type cortical neurons but not of Ptpδ(−/−). Arborization of cortical basal dendrites was reduced in Fyn(−/−) as well as in Ptpδ(+/−); Fyn(+/−) double-heterozygous mutants. Collectively, PTPδ mediates Sema3A-signaling through the activation of Fyn by C-terminal dephosphorylation. SIGNIFICANCE STATEMENT The relation of leukocyte common antigen-related (LAR) class protein tyrosine phosphatases (PTPs) and specific axon guidance cues is poorly defined. We show that PTP-3, a LAR homolog in Caenorhabditis elegans, participates in Sema2A-regulated axon guidance. PTPδ, a member of vertebrate LAR class PTPs, is involved in Sema3A-regulated cortical dendritic growth. In Sema3A signaling, PTPδ activates Fyn and Src kinases by dephosphorylating their C-terminal Tyr residues. This is the first evidence showing that LAR class PTPs participate in Semaphorin signaling in vivo. |
| تدمد: | 1529-2401 0270-6474 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3eeb9bb68b1b613c969032739fdd4f34 https://doi.org/10.1523/jneurosci.2519-16.2017 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3eeb9bb68b1b613c969032739fdd4f34 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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