Antiproliferative effect of a synthetic aptamer mimicking androgen response elements in the LNCaP cell line
| العنوان: | Antiproliferative effect of a synthetic aptamer mimicking androgen response elements in the LNCaP cell line |
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| المؤلفون: | Laleh Shariati, Mina Mirian, A R Einizadeh, Maryam Boshtam, Hossein Khanahmad, M Sojoudi, Ali Rezaei, Leila Darzi, Shirin Kouhpayeh, Zahra Hejazi |
| المصدر: | Cancer Gene Therapy. 23:254-257 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Cancer Research, Cell Survival, medicine.drug_class, Gene Expression, Dehydroepiandrosterone, Biology, Response Elements, Transfection, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, Cell Line, Tumor, LNCaP, medicine, Humans, RNA, Messenger, Receptor, Molecular Biology, Cell Proliferation, Hormone response element, Binding Sites, Base Sequence, Prostatic Neoplasms, Aptamers, Nucleotide, medicine.disease, Androgen, Molecular biology, Androgen receptor, 030104 developmental biology, Receptors, Androgen, 030220 oncology & carcinogenesis, Androgens, Cancer research, Molecular Medicine |
| الوصف: | Prostate cancer usually develops to a hormone-refractory state that is irresponsive to conventional therapeutic approaches. Therefore, new methods for treating aggressive prostate cancer are under development. Because of the importance of androgen receptors (ARs) in the development of the hormone-refractory state and AR mechanism of action, this study was designed. A single-stranded DNA as an aptamer was designed that could mimic the hormone response element (HRE). The LNCaP cells as an AR-rich model were divided into three sets of triplicate groups: the test group was transfected with Aptamer Mimicking HRE (AMH), Mock received only transfection reagents (mock) and a negative control. All three sets received 0, 10 and 100 nM of dehydroepiandrosterone (DHEA) separately. Data analysis showed hormone dependency of LNCaP cells in the negative control group upon treatment with 10 and 100 nM DHEA (compared with cells left untreated (P=0.001)). Transfection of AMH resulted in significant reduction of proliferation in the test group when compared with the negative control group with 10 (P=0.001) or 100 nM DHEA (P=0.02). AMH can form a hairpin structure at 37 °C and mimic the genomic HRE. Hence, it is capable of effectively competing with genomic HRE and interrupting the androgen signaling pathway in a prostate cancer cell line (LNCaP). |
| تدمد: | 1476-5500 0929-1903 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3efacbed5fe8d303a4d284b9cdc17a2e https://doi.org/10.1038/cgt.2016.26 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3efacbed5fe8d303a4d284b9cdc17a2e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765500 09291903 |
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