Functional Intronic Variant in the Retinoblastoma 1 Gene Underlies Broiler Chicken Adiposity by Altering Nuclear Factor-kB and SRY-Related HMG Box Protein 2 Binding Sites
| العنوان: | Functional Intronic Variant in the Retinoblastoma 1 Gene Underlies Broiler Chicken Adiposity by Altering Nuclear Factor-kB and SRY-Related HMG Box Protein 2 Binding Sites |
|---|---|
| المؤلفون: | Hui Li, Yumao Li, Yaofeng Chen, Peng Luan, Bohan Cheng, Xi Chen, Li Leng, Yuhang Sun, Chang Liu, Hui Zhang |
| المصدر: | Journal of Agricultural and Food Chemistry. 67:9727-9737 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, HMG-box, Abdominal Fat, Single-nucleotide polymorphism, Biology, Polymorphism, Single Nucleotide, Retinoblastoma Protein, 01 natural sciences, Avian Proteins, Genotype, Animals, Binding site, Gene, Alleles, SOX Transcription Factors, Adiposity, Binding Sites, 010401 analytical chemistry, NF-kappa B, Intron, General Chemistry, Molecular biology, Introns, eye diseases, 0104 chemical sciences, Testis determining factor, Intronic SNP, General Agricultural and Biological Sciences, Chickens, Protein Binding, 010606 plant biology & botany |
| الوصف: | The present study aimed to search for chicken abdominal fat deposition-related polymorphisms within RB1 and to provide functional evidence for significantly associated genetic variants. Association analyses showed that 11 single nucleotide polymorphisms (SNPs) in intron 17 of RB1, were significantly associated with both abdominal fat weight (P G repressed the transcriptional efficiency of RB1 in vitro, through binding nuclear factor-kappa B (NF-KB) and SRY-related HMG box protein 2 (SOX2). Furthermore, RB1 mRNA expression levels in the abdominal fat tissue of individuals with the A/A genotype of g.32828A>G were lower than those of individuals with the G/G genotype. Collectively, we propose that the intronic SNP g.32828A>G of RB1 is an obesity-associated variant that directly affects binding with NF-KB and SOX2, leading to changes in RB1 expression which in turn may influence chicken abdominal fat deposition. |
| تدمد: | 1520-5118 0021-8561 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f0cf1e96ec1e2438e92845a68f69822 https://doi.org/10.1021/acs.jafc.9b01719 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....3f0cf1e96ec1e2438e92845a68f69822 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205118 00218561 |
|---|