Mechanisms of Nucleosome Reorganization by PARP1
| العنوان: | Mechanisms of Nucleosome Reorganization by PARP1 |
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| المؤلفون: | Sohail Akhtar, John M. Pascal, N. S. Gerasimova, Elena Kotova, Mikhail P. Kirpichnikov, D. K. Nilov, Marie-France Langelier, Sergey Pushkarev, N. V. Maluchenko, Alexey V. Feofanov, Vasily M. Studitsky |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 12127, p 12127 (2021) International Journal of Molecular Sciences Volume 22 Issue 22 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA Repair, DNA repair, QH301-705.5, Poly (ADP-Ribose) Polymerase-1, Molecular Dynamics Simulation, Catalysis, Article, Inorganic Chemistry, Histones, chemistry.chemical_compound, Histone H1, Nucleosome, Humans, Physical and Theoretical Chemistry, Biology (General), Promoter Regions, Genetic, Molecular Biology, QD1-999, Spectroscopy, Regulation of gene expression, Organic Chemistry, nucleosome, Promoter, General Medicine, spFRET microscopy, Linker DNA, Chromatin, molecular dynamics, Computer Science Applications, Cell biology, Nucleosomes, DNA-Binding Proteins, Chemistry, chemistry, Gene Expression Regulation, poly(ADP-ribose) polymerase 1, DNA |
| الوصف: | Poly(ADP-ribose) polymerase 1 (PARP1) is an enzyme involved in DNA repair, chromatin organization and transcription. During transcription initiation, PARP1 interacts with gene promoters where it binds to nucleosomes, replaces linker histone H1 and participates in gene regulation. However, the mechanisms of PARP1-nucleosome interaction remain unknown. Here, using spFRET microscopy, molecular dynamics and biochemical approaches we identified several different PARP1-nucleosome complexes and two types of PARP1 binding to mononucleosomes: at DNA ends and end-independent. Two or three molecules of PARP1 can bind to a nucleosome depending on the presence of linker DNA and can induce reorganization of the entire nucleosome that is independent of catalytic activity of PARP1. Nucleosome reorganization depends upon binding of PARP1 to nucleosomal DNA, likely near the binding site of linker histone H1. The data suggest that PARP1 can induce the formation of an alternative nucleosome state that is likely involved in gene regulation and DNA repair. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f29d93424ff27c62d53d55374d23165 https://www.mdpi.com/1422-0067/22/22/12127 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3f29d93424ff27c62d53d55374d23165 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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