Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in the Ovine Dairy Chain and in Farm-Related Humans
| العنوان: | Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in the Ovine Dairy Chain and in Farm-Related Humans |
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| المؤلفون: | Gilberto Giangolini, Lucia Decastelli, G. Giacinti, Daniela Manila Bianchi, Nicla Marri, D. Sagrafoli, Silvia Gallina, Alberto Bellio, Simonetta Amatiste, Guerrino Macori |
| المصدر: | Toxins, Vol 9, Iss 5, p 161 (2017) Toxins Toxins; Volume 9; Issue 5; Pages: 161 |
| بيانات النشر: | MDPI AG, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Staphylococcus aureus, PFGE, Panton-Valentine leukocidin, MRSA, typing, Farms, Food Safety, Health, Toxicology and Mutagenesis, 030106 microbiology, Bacterial Toxins, Exotoxins, lcsh:Medicine, Food Contamination, Biology, Toxicology, medicine.disease_cause, Article, Microbiology, 03 medical and health sciences, Methicillin, Leukocidins, Pulsed-field gel electrophoresis, medicine, Bulk tank, Animals, Humans, Molecular Epidemiology, Farmers, Sheep, Molecular epidemiology, Transmission (medicine), lcsh:R, biochemical phenomena, metabolism, and nutrition, Anti-Bacterial Agents, Milk, Italy, Genes, Bacterial, Methicillin Resistance, Panton–Valentine leukocidin, Methicillin Susceptible Staphylococcus Aureus, Food contaminant, Environmental Monitoring |
| الوصف: | Staphylococcus aureus is a major cause of clinical infections in humans and its enterotoxins cause foodborne disease. In the present study, we tested a total of 51 isolates of S. aureus from small-ruminant dairy farms with artisan dairy facilities, all located in Latium, Italy. The farms have a known history of a high prevalence of methicillin-resistant S. aureus (MRSA). Most of the MRSA isolates (27 of 51) belonged to spa-type t127 (43.1%), followed by t2678 (3.9%), t044 (2%), t1166 (2%), and t1773 (2%). PFGE performed on mecA positive strains identified one cluster (≥ 80% of similarity), comprising 22 MRSA. Nine of twenty-two MRSA isolates were assigned human host origin, and 13 isolates did not belong to a specific host. During the characterization study, one strain isolated from bulk tank milk samples harbored the pvl gene; the strain was not enterotoxigenic with a non-specific host according to the biotyping scheme, highlighting the possible emerging risk of transmission of bacterial virulence factors by foods, the environment, and foodhandlers. These findings stress the importance of hygienic measures at all processing steps of the food production chain and underline that monitoring for the presence of MRSA throughout the food chain is essential for public health. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6651 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f30d26f1013692a15d7b47bc9983625 http://www.mdpi.com/2072-6651/9/5/161 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3f30d26f1013692a15d7b47bc9983625 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726651 |
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