Proteomic characterisations of ulcerative colitis endoscopic biopsies associate with clinically relevant histological measurements of disease severity
| العنوان: | Proteomic characterisations of ulcerative colitis endoscopic biopsies associate with clinically relevant histological measurements of disease severity |
|---|---|
| المؤلفون: | Aaron M Gruver, Salisha Hill, Daniel C. Liebler, Jochen Schmitz, Juraj Bodo, Keith Lai, Robert J. Benschop, Bradley L. Ackermann, David C Gemperline, Ryan D. Morrison, Eric D. Hsi, Matt D Westfall |
| المصدر: | Journal of Clinical Pathology. 75:636-642 |
| بيانات النشر: | BMJ, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Proteomics, Interleukin-23 receptor, Pathology, medicine.medical_specialty, business.industry, Biopsy, Colonoscopy, General Medicine, Interleukin-17 receptor, medicine.disease, Interleukin-23, Severity of Illness Index, Ulcerative colitis, Pathology and Forensic Medicine, Interleukin 23, Humans, Medicine, Colitis, Ulcerative, Histopathology, Interleukin 17, Intestinal Mucosa, Colitis, business, G alpha subunit |
| الوصف: | Aims and methodsAccurate protein measurements using formalin-fixed biopsies are needed to improve disease characterisation. This feasibility study used targeted and global mass spectrometry (MS) to interrogate a spectrum of disease severities using 19 ulcerative colitis (UC) biopsies.ResultsTargeted assays for CD8, CD19, CD132 (interleukin-2 receptor subunit gamma/common cytokine receptor gamma chain), FOXP3 (forkhead box P3) and IL17RA (interleukin 17 receptor A) were successful; however, assays for IL17A (interleukin 17A), IL23 (p19) (interleukin 23, alpha subunit p19) and IL23R (interleukin 23 receptor) did not permit target detection. Global proteome analysis (4200 total proteins) was performed to identify pathways associated with UC progression. Positive correlation was observed between histological scores indicating active colitis and neutrophil-related measurements (R2=0.42–0.72); inverse relationships were detected with cell junction targets (R2=0.49–0.71) and β-catenin (R2=0.51–0.55) attributed to crypt disruption. An exploratory accuracy assessment with Geboes Score and Robarts Histopathology Index cut-offs produced sensitivities/specificities of 72.7%/75.0% and 100.0%/81.8%, respectively.ConclusionsPathologist-guided MS assessments provide a complementary approach to histological scoring systems. Additional studies are indicated to verify the utility of this novel approach. |
| تدمد: | 1472-4146 0021-9746 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f40e2adf179798c79c03a186d40329c https://doi.org/10.1136/jclinpath-2021-207718 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3f40e2adf179798c79c03a186d40329c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14724146 00219746 |
|---|