Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world

التفاصيل البيبلوغرافية
العنوان: Multi-PK antibodies: Powerful analytical tools to explore the protein kinase world
المؤلفون: Isamu Kameshita, Yasunori Sugiyama
المصدر: Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports, Vol 11, Iss C, Pp 40-45 (2017)
بيانات النشر: Elsevier, 2017.
سنة النشر: 2017
مصطلحات موضوعية: 0301 basic medicine, CNBr, cyanogen bromide, Monoclonal antibody, Proteomics, CaMK, Ca2+/calmodulin-dependent protein kinase, Biophysics, Kinomics, 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis, Computational biology, Review Article, CDKL5, cyclin-dependent kinase-like 5, Biochemistry, Protein kinase, lcsh:Biochemistry, 03 medical and health sciences, Protein phosphorylation, NCK1, lcsh:QD415-436, MeCP2, methylated-CpG-binding protein 2, Protein kinase A, lcsh:QH301-705.5, MAPK14, 030102 biochemistry & molecular biology, biology, Kinase, GRB10, DCLK, double-cortin like protein kinase, IPG, immobilized pH gradient, IEF, isoelectric focusing, 030104 developmental biology, lcsh:Biology (General), CDC37, FAK, focal adhesion kinase, Dnmt1, DNA methyltransferase 1, biology.protein, MAPK, mitogen-activated protein kinase
الوصف: Diverse biological events are regulated through protein phosphorylation mediated by protein kinases. Some of these protein kinases are known to be involved in the pathogenesis of various diseases. Although 518 protein kinase genes were identified in the human genome, it remains unclear how many and what kind of protein kinases are expressed and activated in cells and tissues under varying situations. To investigate cellular signaling by protein kinases, we developed monoclonal antibodies, designated as Multi-PK antibodies, that can recognize multiple protein kinases in various biological species. These Multi-PK antibodies can be used to profile the kinases expressed in cells and tissues, identify the kinases of special interest, and analyze protein kinase expression and phosphorylation state. Here we introduce some applications of Multi-PK antibodies to identify and characterize the protein kinases involved in epigenetics, glucotoxicity in type 2 diabetes, and pathogenesis of ulcerative colitis. In this review, we focus on the recently developed technologies for kinomics studies using the powerful analytical tools of Multi-PK antibodies.
Highlights • Multi-PK antibodies recognize a wide variety of protein kinases. • New analytical methods using Multi-PK antibodies for protein kinase studies are explained. • Kinomics studies using Multi-PK antibodies are introduced.
اللغة: English
تدمد: 2405-5808
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f5b283bbad69dae81fc2b25ceca05c8
http://europepmc.org/articles/PMC5614692
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....3f5b283bbad69dae81fc2b25ceca05c8
قاعدة البيانات: OpenAIRE