Evaluation of Anti-Phytoplasma Properties of Surfactin and Tetracycline Towards Lime Witches’ Broom Disease Using Real-Time PCR
| العنوان: | Evaluation of Anti-Phytoplasma Properties of Surfactin and Tetracycline Towards Lime Witches’ Broom Disease Using Real-Time PCR |
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| المؤلفون: | Hagh Nazari A, Maryam Mousivand, Mohsen Mardi, Nayere Askari, S. Soheilivand, Saeed Abbasalizadeh, Abbas Foroutan, Salehi Jouzani G |
| المصدر: | Journal of Microbiology and Biotechnology. 21:81-88 |
| بيانات النشر: | Journal of Microbiology and Biotechnology, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Citrus, Phytoplasma, Tetracycline, Population, engineering.material, Peptides, Cyclic, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Microbiology, Lipopeptides, chemistry.chemical_compound, medicine, education, Plant Diseases, Lime, education.field_of_study, biology, General Medicine, biology.organism_classification, Bacillales, Anti-Bacterial Agents, chemistry, engineering, Mollicutes, Candidatus, lipids (amino acids, peptides, and proteins), Surfactin, Biotechnology, medicine.drug |
| الوصف: | The anti-phytoplasma activities of surfactin (derived from Iranian native Bacillus subtilis isolates) and tetracycline towards Candidatus "Phytoplasma aurantifolia", the agent of lime Witches' broom disease, were investigated. HPLC was used to quantify the surfactin production in four previously characterized native surfactin-producing strains, and the one producing the highest amount of surfactin (about 1,500 mg/l) was selected and cultivated following optimized production and extraction protocols. Different combinations of purified surfactin and commercial tetracycline were injected into artificially phytoplasma-infected Mexican lime seedlings using a syringe injection system. An absolute quantitative real-time PCR system was developed to monitor the phytoplasma population shifts in the lime phloem during 3 months following the injections. The results revealed that the injections of surfactin or tetracycline had a significant inhibitory effect on Candidatus "P. aurantifolia". However, the combined treatment with both surfactin and tetracycline (1:1) resulted in the highest inhibition due to a synergic effect, which suppressed the phytoplasma population from about 2×10 5 to less than 10 phytoplasma units/g plant tissue. |
| تدمد: | 1738-8872 1017-7825 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fa7dfeb08e45c0e8c795084f83e573f https://doi.org/10.4014/jmb.1007.07047 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3fa7dfeb08e45c0e8c795084f83e573f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17388872 10177825 |
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