Chick Embryo Partial Ischemia Model: A New Approach to Study Ischemia Ex Vivo
| العنوان: | Chick Embryo Partial Ischemia Model: A New Approach to Study Ischemia Ex Vivo |
|---|---|
| المؤلفون: | Jamila H. Siamwala, M. Ilayaraja, Suvro Chatterjee, Syamantak Majumder, Himabindu Reddy Seerapu, Swaraj Sinha |
| المصدر: | PLoS ONE PLoS ONE, Vol 5, Iss 5, p e10524 (2010) |
| بيانات النشر: | Public Library of Science, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Pathology, Time Factors, Ranolazine, lcsh:Medicine, Apoptosis, Chick Embryo, Pharmacology, Piperazines, chemistry.chemical_compound, Ischemia, Creatine Kinase, MB Form, Edema, Cardiovascular Disorders/Vascular Biology, lcsh:Science, Multidisciplinary, Neovascularization, Pathologic, Goats, Embryo, Glutathione, Endothelial stem cell, Health, Organ Specificity, Vascular Surgical Procedures, Cardiovascular Disorders/Myocardial Infarction, medicine.drug, Research Article, medicine.medical_specialty, Cell Survival, Trimetazidine, Creatine, In vivo, medicine, Animals, Cell Proliferation, Cardiovascular Disorders/Pulmonary Vascular Diseases, Myocardium, lcsh:R, Biochemistry/Chemical Biology of the Cell, Endothelial Cells, medicine.disease, Hypoxia-Inducible Factor 1, alpha Subunit, Disease Models, Animal, chemistry, Blood Vessels, lcsh:Q, Acetanilides, Reactive Oxygen Species, Ex vivo, Cardiovascular Disorders/Valvular Disease |
| الوصف: | BACKGROUND: Ischemia is a pathophysiological condition due to blockade in blood supply to a specific tissue thus damaging the physiological activity of the tissue. Different in vivo models are presently available to study ischemia in heart and other tissues. However, no ex vivo ischemia model has been available to date for routine ischemia research and for faster screening of anti-ischemia drugs. In the present study, we took the opportunity to develop an ex vivo model of partial ischemia using the vascular bed of 4(th) day incubated chick embryo. METHODOLOGY/PRINCIPAL FINDINGS: Ischemia was created in chick embryo by ligating the right vitelline artery using sterile surgical suture. Hypoxia inducible factor- 1 alpha (HIF-1alpha), creatine phospho kinase-MB and reactive oxygen species in animal tissues and cells were measured to confirm ischemia in chick embryo. Additionally, ranolazine, N-acetyl cysteine and trimetazidine were administered as an anti-ischemic drug to validate the present model. Results from the present study depicted that blocking blood flow elevates HIF-1alpha, lipid peroxidation, peroxynitrite level in ischemic vessels while ranolazine administration partially attenuates ischemia driven HIF-1alpha expression. Endothelial cell incubated on ischemic blood vessels elucidated a higher level of HIF-1alpha expression with time while ranolazine treatment reduced HIF-1alpha in ischemic cells. Incubation of caprine heart strip on chick embryo ischemia model depicted an elevated creatine phospho kinase-MB activity under ischemic condition while histology of the treated heart sections evoked edema and disruption of myofibril structures. CONCLUSIONS/SIGNIFICANCE: The present study concluded that chick embryo partial ischemia model can be used as a novel ex vivo model of ischemia. Therefore, the present model can be used parallel with the known in vivo ischemia models in understanding the mechanistic insight of ischemia development and in evaluating the activity of anti-ischemic drug. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fbc217170c4a9537371495baabb86de http://europepmc.org/articles/PMC2866318 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....3fbc217170c4a9537371495baabb86de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|