Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies
| العنوان: | Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies |
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| المؤلفون: | Ericka Kirkpatrick, Seth J. Zost, Michael J. Hope, Florian Krammer, Katalin Karikó, Drew Weissman, Scott E. Hensley, Christopher Barbosa, Kaela Parkhouse, Ying K. Tam, Thomas D. Madden, Barbara L. Mui, Meagan McMahon, Norbert Pardi |
| المصدر: | Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, Science, General Physics and Astronomy, Heterologous, Hemagglutinin (influenza), Enzyme-Linked Immunosorbent Assay, Biology, medicine.disease_cause, Antibodies, Viral, General Biochemistry, Genetics and Molecular Biology, Virus, Antigenic drift, Article, Madin Darby Canine Kidney Cells, 03 medical and health sciences, Mice, Dogs, Influenza A virus, medicine, Animals, RNA, Messenger, lcsh:Science, Cells, Cultured, Phylogeny, Mice, Inbred BALB C, Multidisciplinary, Influenza A Virus, H5N1 Subtype, Ferrets, RNA, virus diseases, General Chemistry, Flow Cytometry, Orthomyxoviridae, Virology, 3. Good health, 030104 developmental biology, Hemagglutinins, Immunization, biology.protein, lcsh:Q, Female, Rabbits, Antibody |
| الوصف: | Currently available influenza virus vaccines have inadequate effectiveness and are reformulated annually due to viral antigenic drift. Thus, development of a vaccine that confers long-term protective immunity against antigenically distant influenza virus strains is urgently needed. The highly conserved influenza virus hemagglutinin (HA) stalk represents one of the potential targets of broadly protective/universal influenza virus vaccines. Here, we evaluate a potent broadly protective influenza virus vaccine candidate that uses nucleoside-modified and purified mRNA encoding full-length influenza virus HA formulated in lipid nanoparticles (LNPs). We demonstrate that immunization with HA mRNA-LNPs induces antibody responses against the HA stalk domain of influenza virus in mice, rabbits, and ferrets. The HA stalk-specific antibody response is associated with protection from homologous, heterologous, and heterosubtypic influenza virus infection in mice. The highly conserved influenza virus hemagglutinin (HA) stalk represents a potential target for a broadly protective vaccine. Here, the authors show that immunization with nucleoside-modified mRNA encoding full-length HA formulated in lipid nanoparticles elicits HA stalk-specific antibodies and protects from heterosubtypic virus infection. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::400e70caa6ddd52ede0804ed2bbeac15 https://doaj.org/article/5342eb93bec04c388435188e0f3081ae |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....400e70caa6ddd52ede0804ed2bbeac15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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