miR-204–containing exosomes ameliorate GVHD-associated dry eye disease
| العنوان: | miR-204–containing exosomes ameliorate GVHD-associated dry eye disease |
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| المؤلفون: | Tian Zhou, Chang He, Peilong Lai, Ziqi Yang, Yan Liu, Huiyi Xu, Xiaojing Lin, Biyan Ni, Rong Ju, Wei Yi, Lingyi Liang, Duanqing Pei, Charles E. Egwuagu, Xialin Liu |
| المصدر: | Science Advances |
| بيانات النشر: | American Association for the Advancement of Science, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Multidisciplinary, genetic structures, SciAdv r-articles, Diseases and Disorders, sense organs, Biomedicine and Life Sciences, Health and Medicine, eye diseases, Research Article |
| الوصف: | Description MSC-exo eye drops alleviate GVHD-associated dry eye disease by shifting M1 macrophages to M2 via miR-204. Graft-versus-host disease (GVHD)–associated dry eye disease is characterized by extensive inflammatory destruction in the ocular surface and causes unbearable pain and visual impairment. Current treatments provide limited benefits. Here, we report that exosomes from mesenchymal stromal cells (MSC-exo) administered as eye drops notably alleviate GVHD-associated dry eye disease by suppressing inflammation and improving epithelial recovery in mice and humans. In a prospective clinical trial, 28 eyes with refractory GVHD–dry eye disease exhibited substantial relief after MSC-exo treatment, showing reduced fluorescein scores, longer tear-film breakup time, increased tear secretion, and lower OSDI scores. Mechanistically, MSC-exo reprogramed proinflammatory M1 macrophages toward the immunosuppressive M2 via miR-204–mediated targeting of the IL-6/IL-6R/Stat3 pathway. Blockade of miR-204 abolished the effects of MSC-exo, while overloading L929-exo with miR-204 markedly attenuated dry eye. Thus, this study suggests that MSC-exo are efficacious in treating GVHD-associated dry eye disease and highlights miR-204 as a potential therapeutic agent. |
| اللغة: | English |
| تدمد: | 2375-2548 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::401328cb46b42ad80eb548e2bc083316 http://europepmc.org/articles/PMC8754411 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....401328cb46b42ad80eb548e2bc083316 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23752548 |
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