Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface
| العنوان: | Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface |
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| المؤلفون: | Nathan Klejwa, Kent Kemmish, Adam H. Marblestone, Mark Hamalainen, William Andregg, Andrew Bleloch, Heather Stark, Ken Schatz, George M. Church, Wolfgang Lehrach, Charlotte Bowell, Michael Andregg, Andrew C. Payne, Christopher S. Own |
| المصدر: | PLoS ONE, Vol 8, Iss 7, p e69058 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Technology, lcsh:Medicine, Condensed Matter, 02 engineering and technology, Engineering, Nanotechnology, Nanobiotechnology, lcsh:Science, Condensed-Matter Physics, chemistry.chemical_classification, 0303 health sciences, Multidisciplinary, Physics, Air, Robotics, Polymer, 021001 nanoscience & nanotechnology, Condensed Matter Fluids, Nanorobotics, Solutions, Membrane, Transmission electron microscopy, Microtechnology, Optoelectronics, 0210 nano-technology, Research Article, Materials science, Surface Properties, Materials Science, DNA, Single-Stranded, Bioengineering, Biomaterials, 03 medical and health sciences, Molecule, Thin film, 030304 developmental biology, business.industry, Mechanical Engineering, lcsh:R, Reproducibility of Results, DNA, Microscopy, Electron, Microscopy, Fluorescence, chemistry, Nanoengineering, Bionanotechnology, Biophysics, Nucleic Acid Conformation, lcsh:Q, Nanometre, Threading (protein sequence), business |
| الوصف: | We present "molecular threading", a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4017f6d01e790a8a1c7b6af3822e49ed https://doi.org/10.1371/journal.pone.0069058 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4017f6d01e790a8a1c7b6af3822e49ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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