Characterization of genetically inactivated pertussis toxin mutants: candidates for a new vaccine against whooping cough
| العنوان: | Characterization of genetically inactivated pertussis toxin mutants: candidates for a new vaccine against whooping cough |
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| المؤلفون: | Giacomo Matteucci, A Di Tommaso, M Bugnoli, F Giovannoni, Luciano Nencioni, Roberto Manetti, I Marsili, D Nucci, T De Magistris, M Pizza |
| المصدر: | Infection and Immunity. 58:1308-1315 |
| بيانات النشر: | American Society for Microbiology, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Bordetella pertussis, Whooping Cough, medicine.drug_class, Immunology, Dose-Response Relationship, Immunologic, Pertussis toxin, Monoclonal antibody, medicine.disease_cause, Microbiology, Mice, Adjuvants, Immunologic, In vivo, Formaldehyde, medicine, Animals, Virulence Factors, Bordetella, Amino Acids, Whooping cough, Vaccines, Vaccines, Synthetic, biology, Toxin, Antibodies, Monoclonal, Biological activity, biology.organism_classification, medicine.disease, Antibodies, Bacterial, Infectious Diseases, Pertussis Toxin, biology.protein, Electrophoresis, Polyacrylamide Gel, Parasitology, Antibody, Research Article |
| الوصف: | the introduction of two amino acid substitutions within the enzymatically active subunit S1 of pertussis toxin (PT) abolishes its ADP-ribosyltransferase activity and toxicity on CHO cells (Pizza et al., Science 246:497-500, 1989). These genetically inactivated molecules are also devoid of other in vivo adverse reactions typical of PT, such as induction of leukocytosis, potentiation of anaphylaxis, stimulation of insulin secretion, and histamine sensitivity. However, the mutant PT molecules are indistinguishable from wild-type PT in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and maintain all the physical and chemical properties of PT, including affinity for toxin-neutralizing poly- and monoclonal antibodies. Either alone or stabilized with formaldehyde, PT mutants are able to induce high levels of neutralizing antibodies and to protect mice in a dose-dependent fashion against intracerebral challenge with virulent B. pertussis. These results clearly show that these genetically inactivated PT molecules are nontoxic but still immunogenic and justify their development as a component of a new, safer acellular vaccine against whooping cough. |
| تدمد: | 1098-5522 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::407bc128ac16fb6d350097cf161eafa7 https://doi.org/10.1128/iai.58.5.1308-1315.1990 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....407bc128ac16fb6d350097cf161eafa7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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