Perturbations from thunderstorms can play a notable role in the dynamics of the ionosphere. In this work, ionospheric perturbation effects due to thunderstorms were extracted and studied. Thunderstorm-associated lightning activities and their locations were detected by the World-Wide Lightning Location Network (WWLLN). The mechanical components of ionospheric perturbations due to thunderstorms were extracted from the total electron content (TEC), which was measured at selected thunderstorm locations using the polynomial filtering method. Further analyses were conducted using wavelet analysis and Discrete Fourier Transform (DFT) to study the frequency modes and periodicities of TEC deviation. It was revealed that the highest magnitudes of TEC deviations could reach up to ~2.2 TECUs, with dominant modes of frequency in the range of ~0.2 mHz to ~1.2 mHz, falling within the gravity wave range and the second dominant mode in the acoustic range of >1 mHz to
