Cardosin A endocytosis mediated by integrin leads to lysosome leakage and apoptosis of epithelial cells
| العنوان: | Cardosin A endocytosis mediated by integrin leads to lysosome leakage and apoptosis of epithelial cells |
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| المؤلفون: | Carlos Faro, Pedro Castanheira, Hao Wu, Jordan Tang |
| المصدر: | Proteins: Structure, Function, and Bioinformatics. 87:502-511 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Integrins, Endosome, medicine.medical_treatment, Molecular Sequence Data, Cell, Integrin, Apoptosis, Endocytosis, Biochemistry, Protein Structure, Secondary, 03 medical and health sciences, Structural Biology, Lysosome, Candida albicans, medicine, Humans, Amino Acid Sequence, Molecular Biology, 030304 developmental biology, RGD motif, 0303 health sciences, Protease, biology, Chemistry, 030302 biochemistry & molecular biology, Epithelial Cells, Epithelium, Cell biology, medicine.anatomical_structure, A549 Cells, biology.protein, Lysosomes |
| الوصف: | Cardosin A is an aspartic protease present in large amount in the pistils of cardoon flowers. This protease is known to contain an -Arg-Gly-Asp- (RGD) motif located on the molecular surface. In this study, we found that isolated recombinant cardosin A attached to human epithelial cells A549, mediated by the binding of its RGD motif to cell surface integrins. The cell bound cardosin A was internalized to endosomes and lysosomes and triggered the permeability of lysosomal membrane leading to apoptosis of the epithelial cells. These events are identical to those observed for three RGD-containing aspartic proteases, Saps 4-6, secreted by Candida albicans. Such a process, which has been called the Trojan Horse mechanism, is believed to benefit the invasion of C. albican into the epithelium of the host. The location of the RGD motifs of cardosin A and Saps 4-6 are on the opposite ends of the homologous three-dimensional structures, suggesting that the Trojan Horse mechanism is insensitive to the RGD position. Current finding also suggests that cardosin A may have a defensive function against the ingestion of cardoon flowers by human, insects, and other herbivores. |
| تدمد: | 1097-0134 0887-3585 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::410a358cc539cf809c4b9e29f8a56305 https://doi.org/10.1002/prot.25672 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....410a358cc539cf809c4b9e29f8a56305 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970134 08873585 |
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