Genetic heterogeneity of the NS3 protease gene in hepatitis C virus genotype 1 from untreated infected patients
| العنوان: | Genetic heterogeneity of the NS3 protease gene in hepatitis C virus genotype 1 from untreated infected patients |
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| المؤلفون: | Sophie Vallet, S. Gouriou, Jean-Baptiste Nousbaum, M. C. Legrand-Quillien, Bertrand Picard, Alain Goudeau |
| المصدر: | Journal of Medical Virology. 75:528-537 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, Models, Molecular, Genotype, medicine.medical_treatment, Hepatitis C virus, Molecular Sequence Data, Hepacivirus, Viral quasispecies, Viral Nonstructural Proteins, Biology, medicine.disease_cause, Virus, Virology, medicine, Humans, Amino Acid Sequence, Gene, Genetics, NS3, Protease, Genetic heterogeneity, Genetic Variation, virus diseases, Sequence Analysis, DNA, Middle Aged, Hepatitis C, Infectious Diseases, Mutation, Female |
| الوصف: | De ´partement de Microbiologie Medicale et Mole´culaire, Universite´ Franc¸ois Rabelais, Tours, FranceNS3 protease is essential for hepatitis C Virus(HCV) replication, and is one of the most promis-ing targets for specific anti-HCV therapy. Itsnatural polymorphism has not been studied atthe quasispecies level. In the present work, thegenetic heterogeneity of the NS3 protease genewasanalyzedin17HCVgenotype1(5subtypes1aand 12 subtypes 1b) samples collected frominfectedpatientsbeforeanti-viraltherapy.Atotalof 294 clones were sequenced. Although theprotease NS3 is considered to be one of the lessvariable genes in the HCV genome, variability ofboth nucleotide and amino acid sequences wasfound. In variants belonging to 1a and 1b sub-types, 224 and 267 of 543 positions showed oneor more nucleotide substitutions, respectively.Fortyand 74of the181NS3aminoacid positionsshowedatleastonemutationinHCV-1aandHCV-1bisolates,respectively.Mostsubstitutionswereconservative. This substantial polymorphism ofthe NS3 protease produced by HCV-1a and HCV-1b suggests that, despite the numerous func-tional and structural constraints, the enzymeis sufficiently flexible to tolerate substitutions.J. Med. Virol. 75:528–537, 2005. |
| تدمد: | 1096-9071 0146-6615 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::413aa05e503ae52bec87d90b50d8ae80 https://doi.org/10.1002/jmv.20302 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....413aa05e503ae52bec87d90b50d8ae80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969071 01466615 |
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