The Nance–Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology
| العنوان: | The Nance–Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology |
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| المؤلفون: | Hao R. Tang, Laura M. Machesky, Alison J. Hardcastle, Margherita Coccia, Michael E. Cheetham, Simon P. Brooks, Maryse Bailly, Naheed Kanuga |
| المصدر: | Human Molecular Genetics |
| بيانات النشر: | Oxford University Press, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Molecular Sequence Data, Arp2/3 complex, macromolecular substances, Cell morphology, 03 medical and health sciences, 0302 clinical medicine, Genetics, Animals, Humans, Amino Acid Sequence, Pseudopodia, Cytoskeleton, Molecular Biology, Genetics (clinical), Actin, health care economics and organizations, 030304 developmental biology, 0303 health sciences, Focal Adhesions, biology, Actin remodeling, Membrane Proteins, Nuclear Proteins, General Medicine, Articles, Actin cytoskeleton, Actins, Cell biology, Protein Structure, Tertiary, Rats, Wiskott-Aldrich Syndrome Protein Family, Gene Knockdown Techniques, biology.protein, Lamellipodium, Caco-2 Cells, 030217 neurology & neurosurgery |
| الوصف: | Nance-Horan syndrome (NHS) is an X-linked developmental disorder, characterized by bilateral congenital cataracts, dental anomalies, facial dysmorphism and mental retardation. Null mutations in a novel gene, NHS, cause the syndrome. The NHS gene appears to have multiple isoforms as a result of alternative transcription, but a cellular function for the NHS protein has yet to be defined. We describe NHS as a founder member of a new protein family (NHS, NHSL1 and NHSL2). Here, we demonstrate that NHS is a novel regulator of actin remodelling and cell morphology. NHS localizes to sites of cell-cell contact, the leading edge of lamellipodia and focal adhesions. The N-terminus of isoforms NHS-A and NHS-1A, implicated in the pathogenesis of NHS, have a functional WAVE homology domain that interacts with the Abi protein family, haematopoietic stem/progenitor cell protein 300 (HSPC300), Nap1 and Sra1. NHS knockdown resulted in the disruption of the actin cytoskeleton. We show that NHS controls cell morphology by maintaining the integrity of the circumferential actin ring and controlling lamellipod formation. NHS knockdown led to a striking increase in cell spreading. Conversely, ectopic overexpression of NHS inhibited lamellipod formation. Remodelling of the actin cytoskeleton and localized actin polymerization into branched actin filaments at the plasma membrane are essential for mediating changes in cell shape, migration and cell contact. Our data identify NHS as a new regulator of actin remodelling. We suggest that NHS orchestrates actin regulatory protein function in response to signalling events during development. |
| اللغة: | English |
| تدمد: | 1460-2083 0964-6906 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4160dc799ac9827f14b96168ea0e1a12 http://europepmc.org/articles/PMC2876887 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4160dc799ac9827f14b96168ea0e1a12 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602083 09646906 |
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