Scratching below the Surface: Wound Healing and Alanine Mutagenesis Provide Unique Insights into Interactions between Eristostatin, Platelets and Melanoma Cells
| العنوان: | Scratching below the Surface: Wound Healing and Alanine Mutagenesis Provide Unique Insights into Interactions between Eristostatin, Platelets and Melanoma Cells |
|---|---|
| المؤلفون: | Mary Ann McLane, Xiaoming Zhang, Apoorva Srivastava, Claire Zelinskas, Jing Tian, Carrie Paquette-Straub, Brett David Hensley |
| المصدر: | Pathophysiology of Haemostasis and Thrombosis. 34:164-168 |
| بيانات النشر: | S. Karger AG, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Blood Platelets, Integrins, Disintegrins, Integrin, Viper Venoms, law.invention, law, Cell Line, Tumor, Physiology (medical), Disintegrin, Animals, Humans, Platelet, Phosphorylation, Melanoma, Alanine, Wound Healing, Binding Sites, biology, Chemistry, Mutagenesis, Hematology, Cell biology, Biochemistry, biology.protein, Recombinant DNA, Peptides, Wound healing |
| الوصف: | To study the molecular mechanism of the disintegrin eristostatin, cellular functional studies were performed using ten recombinant alanine mutants. ADP-induced platelet aggregation revealed critical contributions of seven residues within the ‘RGD loop’ (R24, R27, G28, N31) and C-terminus (W47, N48, G49) of this disintegrin. Using an in vitro scratch wound healing assay, four human melanoma cell lines yielded similar results when exposed to wildtype eristostatin. All eristostatin-treated cells healed less of the wounded area than control conditions. This phenomenon was reproduced when using fibronectin as the matrix. C8161 cells showed significant delay in wound closure with the N-terminal mutant P4A but not with R24A or G28A. Evidence from our laboratory and others suggests neither alpha IIb, alpha 4 nor alpha 5 integrins are directly involved in eristostatin’s interactions. Eristostatin did not affect the number of melanoma cells in culture after 24 h or the development of apoptosis. However, phosphorylation studies performed after these melanoma cells were exposed to eristostatin revealed changes in several tyrosine phosphorylated molecules. |
| تدمد: | 1424-8840 1424-8832 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4176dca0bbe442e9139a892907ddbde2 https://doi.org/10.1159/000092417 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4176dca0bbe442e9139a892907ddbde2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14248840 14248832 |
|---|