Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii
| العنوان: | Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii |
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| المؤلفون: | Damla D. Bilgin, Adrián López García de Lomana, Nitin S. Baliga, Mónica V. Orellana, Saheed Imam, Serdar Turkarslan, Warren Carter, Sascha Schäuble, Nathan D. Price, David J Reiss, Christopher B. Yohn, Jacob J. Valenzuela |
| المصدر: | López García de Lomana, A; Schäuble, S; Valenzuela, J; Imam, S; Carter, W; Bilgin, DD; et al.(2015). Transcriptional program for nitrogen starvation-induced lipid accumulation in Chlamydomonas reinhardtii. Biotechnology for Biofuels, 8(1). doi: 10.1186/s13068-015-0391-z. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/90w4c37c Biotechnology for biofuels, vol 8, iss 1 Biotechnology for Biofuels |
| بيانات النشر: | eScholarship, University of California, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Systems biology, Chlamydomonas reinhardtii, Metabolic network, Lipid accumulation, Management, Monitoring, Policy and Law, Protein degradation, Network modeling, Phenotypic transition, Applied Microbiology and Biotechnology, Industrial Biotechnology, Downregulation and upregulation, Genetics, 2. Zero hunger, biology, Renewable Energy, Sustainability and the Environment, Research, Metabolism, Chemical Engineering, biology.organism_classification, General Energy, Biochemistry, Signal transduction, Intracellular, Transcriptional regulatory network, Biotechnology |
| الوصف: | Background Algae accumulate lipids to endure different kinds of environmental stresses including macronutrient starvation. Although this response has been extensively studied, an in depth understanding of the transcriptional regulatory network (TRN) that controls the transition into lipid accumulation remains elusive. In this study, we used a systems biology approach to elucidate the transcriptional program that coordinates the nitrogen starvation-induced metabolic readjustments that drive lipid accumulation in Chlamydomonas reinhardtii. Results We demonstrate that nitrogen starvation triggered differential regulation of 2147 transcripts, which were co-regulated in 215 distinct modules and temporally ordered as 31 transcriptional waves. An early-stage response was triggered within 12 min that initiated growth arrest through activation of key signaling pathways, while simultaneously preparing the intracellular environment for later stages by modulating transport processes and ubiquitin-mediated protein degradation. Subsequently, central metabolism and carbon fixation were remodeled to trigger the accumulation of triacylglycerols. Further analysis revealed that these waves of genome-wide transcriptional events were coordinated by a regulatory program orchestrated by at least 17 transcriptional regulators, many of which had not been previously implicated in this process. We demonstrate that the TRN coordinates transcriptional downregulation of 57 metabolic enzymes across a period of nearly 4 h to drive an increase in lipid content per unit biomass. Notably, this TRN appears to also drive lipid accumulation during sulfur starvation, while phosphorus starvation induces a different regulatory program. The TRN model described here is available as a community-wide web-resource at http://networks.systemsbiology.net/chlamy-portal. Conclusions In this work, we have uncovered a comprehensive mechanistic model of the TRN controlling the transition from N starvation to lipid accumulation. The program coordinates sequentially ordered transcriptional waves that simultaneously arrest growth and lead to lipid accumulation. This study has generated predictive tools that will aid in devising strategies for the rational manipulation of regulatory and metabolic networks for better biofuel and biomass production. Electronic supplementary material The online version of this article (doi:10.1186/s13068-015-0391-z) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41e54546757eb6b02086c3dc0b081e6f http://www.escholarship.org/uc/item/90w4c37c |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....41e54546757eb6b02086c3dc0b081e6f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |