Comparison of sevenBlastomyces dermatitidisantigens for the detection of antibodies in humans with occupationally acquired blastomycosis
| العنوان: | Comparison of sevenBlastomyces dermatitidisantigens for the detection of antibodies in humans with occupationally acquired blastomycosis |
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| المؤلفون: | R. C. Axtell, Pahresah Roomiany, Gene M. Scalarone |
| المصدر: | Mycoses. 45:282-286 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Antigens, Fungal, Pulmonary disease, Enzyme-Linked Immunosorbent Assay, Dermatology, Medical care, Blastomycosis, Microbiology, Serology, Dogs, Antigen, Animals, Humans, Medicine, Antibodies, Fungal, Mycosis, biology, business.industry, Blastomyces dermatitidis, General Medicine, biology.organism_classification, medicine.disease, Occupational Diseases, Infectious Diseases, Immunology, Blastomyces, Cats, biology.protein, Antibody, business |
| الوصف: | Yeast phase lysate antigens, prepared from seven isolates of Blastomyces dermatitidis (dogs, T-58, M-98; human, Le; soil, S; sea lion, SL; polar bear, PB; cat, C) were assayed by enzyme-linked immunosorbent assay (ELISA) to detect antibodies in human sera. The sera were from individuals potentially exposed to B. dermatitidis while working on a prairie dog relocation project in Colorado. All antigens exhibited greatest reactivity with three specimens in two separate assays. Absorbance values ranged from 0.370 (M-98) to 0.427 (LE) (Trial 1) and from 0.579 (M-98) to 0.714 (PB) (Trial 2) with serum 2; from 0.368 (C) to 0.453 (LE) (Trial 1) and from 0.565 (SL) to 0.694 (S) (Trial 2) with serum 3 and from 0.392 (M-98) to 0.506 (LE) (Trial 1) and from 0.557 (SL) to 0.758 (T-58) (Trial 2) with serum 6. The greatest reactivity was observed with sera from two persons (2 and 3 from the same individual and serum 6) who became ill with symptoms of pulmonary disease and sought medical care. Both patients were subsequently diagnosed with blastomycosis by microscopic and culture methods. The study indicated that all of the lysate antigens, regardless of source, could be used to reliably diagnose blastomycosis. |
| تدمد: | 1439-0507 0933-7407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42263b3a419cf8badb2c7bf41f9ab9ed https://doi.org/10.1046/j.1439-0507.2002.00764.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....42263b3a419cf8badb2c7bf41f9ab9ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390507 09337407 |
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