Regeneration in Experimental Alveolar Bone Defect Using Human Umbilical Cord Mesenchymal Stem Cells
| العنوان: | Regeneration in Experimental Alveolar Bone Defect Using Human Umbilical Cord Mesenchymal Stem Cells |
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| المؤلفون: | Naoto Suda, Mikiko Mano, Rei Shinagawa, Kazuyuki Tokioka, Akiko Toyota |
| المصدر: | Cell Transplantation, Vol 30 (2021) Cell Transplantation |
| بيانات النشر: | SAGE Publishing, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Pathology, medicine.medical_specialty, Bone Regeneration, umbilical cord mesenchymal stem cells, Biomedical Engineering, lcsh:Medicine, Regenerative medicine, Umbilical cord, Umbilical Cord, 03 medical and health sciences, 0302 clinical medicine, SOX2, Osteogenesis, medicine, enzymatic digestion, Humans, Osteopontin, Dental alveolus, Transplantation, biology, Chemistry, Regeneration (biology), Mesenchymal stem cell, lcsh:R, magnetic-activated cell sorting, Cell Differentiation, Mesenchymal Stem Cells, 030206 dentistry, Cell Biology, 030104 developmental biology, medicine.anatomical_structure, alveolar cleft, biology.protein, Original Article |
| الوصف: | Cleft lip and palate is a congenital disorder including cleft lip, and/or cleft palate, and/or alveolar cleft, with high incidence.The alveolar cleft causes morphological and functional abnormalities. To obtain bone bridge formation and continuous structure between alveolar clefts, surgical interventions are performed from infancy to childhood. However, desirable bone bridge formation is not obtained in many cases. Regenerative medicine using mesenchymal stem cells (MSCs) is expected to be a useful strategy to obtain sufficient bone bridge formation between alveolar clefts. In this study, we examined the effect of human umbilical cord-derived MSCs by transplantation into a rat experimental alveolar cleft model. Human umbilical cords were digested enzymatically and the isolated cells were collected (UC-EZ cells). Next, CD146-positive cells were enriched from UC-EZ cells by magnetic-activated cell sorting (UC-MACS cells). UC-EZ and UC-MACS cells showed MSC gene/protein expression, in vitro. Both cells had multipotency and could differentiate to osteogenic, chondrogenic, and adipogenic lineages under the differentiation-inducing media. However, UC-EZ cells lacked Sox2 expression and showed the lower ratio of MSCs than UC-MACS cells. Thus, UC-MACS cells were transplanted with hydroxyapatite and collagen (HA + Col) into alveolar cleft model to evaluate bone formation in vivo. The results of micro computed tomography and histological staining showed that UC-MACS cells with HA + Col induced more abundant bone formation between the experimental alveolar clefts than HA + Col implantation only. Cells immunopositive for osteopontin were accumulated along the bone surface and some of them were embedded in the bone. Cells immunopositive for human-specific mitochondria were aligned along the newly formed bone surface and in the new bone, suggesting that UC-MACS cells contributed to the bone bridge formation between alveolar clefts. These findings indicate that human umbilical cords are reliable bioresource and UC-MACS cells are useful for the alveolar cleft regeneration. |
| اللغة: | English |
| تدمد: | 1555-3892 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::427ef54328de671f569911dfb5435626 https://doaj.org/article/11a7f601d3054e238ec8b88083a30703 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....427ef54328de671f569911dfb5435626 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15553892 |
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