Comprehensive analysis of a dipeptide library to identify ghrelin release‐modulating peptides
| العنوان: | Comprehensive analysis of a dipeptide library to identify ghrelin release‐modulating peptides |
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| المؤلفون: | Hayato Aoki, Hiroshi Iwakura, Kazuo Inoue, Shigenobu Matsumura, Yuki Tokuyama, Kousaku Ohinata, Junya Nakato, Yuta Yamamoto |
| المصدر: | FEBS Letters. 593:2637-2645 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, MAPK/ERK pathway, Food intake, Biophysics, Pharmacology, Biochemistry, Eating, Mice, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, In vivo, Cell Line, Tumor, Genetics, Animals, Molecular Biology, 030304 developmental biology, 0303 health sciences, Dipeptide, Chemistry, digestive, oral, and skin physiology, 030302 biochemistry & molecular biology, Dipeptides, Cell Biology, Ghrelin, In vitro, Cell culture, hormones, hormone substitutes, and hormone antagonists, Ghrelin secretion |
| الوصف: | We performed a comprehensive analysis of ghrelin release-modulating activity of a dipeptide library using MGN3-1, a ghrelin-producing cell line. We found that most dipeptides suppress ghrelin secretion, whereas the N-terminal Ser-containing dipeptides and a few others stimulate it. N-terminal amino acid residues, but not C-terminal residues, play a dominant role in the effects of dipeptides. Among dipeptides, Leu-Ile (LI) and Ser-Val (SV) most strongly suppress and stimulate ghrelin secretion, respectively. LI activates Gi signaling and SV acts via the MAPK pathway. Orally administered LI and SV reduce and increase plasma ghrelin levels and food intake in mice, respectively. In conclusion, LI and SV, found based on the comprehensive screening of a dipeptide library, modulate ghrelin secretion in vitro and in vivo. |
| تدمد: | 1873-3468 0014-5793 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4290d7378eaedb1c0754835f1d7d7d97 https://doi.org/10.1002/1873-3468.13522 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4290d7378eaedb1c0754835f1d7d7d97 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733468 00145793 |
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