The Nucleotide Sequence of Koala ( Phascolarctos cinereus ) Retrovirus: a Novel Type C Endogenous Virus Related to Gibbon Ape Leukemia Virus
| العنوان: | The Nucleotide Sequence of Koala ( Phascolarctos cinereus ) Retrovirus: a Novel Type C Endogenous Virus Related to Gibbon Ape Leukemia Virus |
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| المؤلفون: | Wayne F. Robinson, Lindell Bromham, Tracy M. O'Brien, Jon Hanger, Jeff J. McKee |
| المصدر: | Journal of Virology. 74:4264-4272 |
| بيانات النشر: | American Society for Microbiology, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | viruses, Molecular Sequence Data, Immunology, Endogenous retrovirus, Biology, Microbiology, Retrovirus, Virology, Animals, Phylogeny, Southern blot, Base Sequence, RNA-Directed DNA Polymerase, Endogenous Retroviruses, Nucleic acid sequence, Provirus, biology.organism_classification, Molecular biology, genomic DNA, Marsupialia, Leukemia Virus, Gibbon Ape, Insect Science, DNA, Viral, RNA, Viral, Recombination and Evolution, Koala retrovirus |
| الوصف: | A novel retrovirus, morphologically consistent with mammalian C-type retroviruses, was detected by electron microscopy in mitogen-stimulated peripheral blood mononuclear cell cultures from 163 koalas and in lymphoma tissue from 3 koalas. PCR amplified provirus from the blood and tissues of 17 wild and captive koalas, and reverse transcriptase-PCR demonstrated viral mRNA, viral genomic RNA, and reverse transcriptase activity in koala serum and cell culture supernatants. Comparison of viral sequences derived from genomic DNA and mRNA showed identity indicative of a single retroviral species—here designated koala retrovirus (KoRV). Southern blot analysis of koala tissue genomic DNA using labelled KoRV probes demonstrated banding consistent with an endogenous retrovirus. Complete and apparently truncated proviruses were detected in DNA of both clinically normal koalas and those with hematopoietic disease. KoRV-related viruses were not detected in other marsupials, and phylogenetic analysis showed that KoRV paradoxically clusters with gibbon ape leukemia virus (GALV). The strong similarity between GALV and KoRV suggests that these viruses are closely related and that recent cross-host transmission has occurred. The complete proviral DNA sequence of KoRV is reported. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42de956512eb91919134ac956718d180 https://doi.org/10.1128/jvi.74.9.4264-4272.2000 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....42de956512eb91919134ac956718d180 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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