Dual culture of atoxigenic and toxigenic strains of Aspergillus flavus to gain insight into repression of aflatoxin biosynthesis and fungal interaction
| العنوان: | Dual culture of atoxigenic and toxigenic strains of Aspergillus flavus to gain insight into repression of aflatoxin biosynthesis and fungal interaction |
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| المؤلفون: | Sui Sheng T. Hua, Gaganjot Sidhu, Siov Bouy L. Sarreal, Dan E. Parfitt |
| المصدر: | Mycotoxin Research. 35:381-389 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Microbiological Techniques, Aflatoxin, Hypha, Genes, Fungal, Aspergillus flavus, Biology, Toxicology, 01 natural sciences, Microbiology, Agar plate, 03 medical and health sciences, Aflatoxins, Gene cluster, Gene expression, heterocyclic compounds, Psychological repression, Gene, 0303 health sciences, 030302 biochemistry & molecular biology, 010401 analytical chemistry, food and beverages, biology.organism_classification, 0104 chemical sciences, Agar, Multigene Family, Microbial Interactions, Biotechnology |
| الوصف: | Application of atoxigenic strains to compete against toxigenic strains of Aspergillus flavus strains has emerged as one of the practical strategies for reducing aflatoxin contamination in corn, peanut, and tree nuts. The actual mechanism that results in aflatoxin reduction is not fully understood. Real-time RT-PCR and relative quantification of gene expression protocol were applied to elucidate the molecular mechanism. Transcriptional analyses of aflatoxin biosynthetic gene cluster in dual culture of toxigenic and atoxigenic A. flavus strains were carried out. Six targeted genes, aflR, aflJ, omtA, ordA, pksA, and vbs, were downregulated to variable levels depending on paired strains of toxigenic and atoxigenic A. flavus. Consistent with the decreased gene expression levels, the aflatoxin concentrations in dual cultures were reduced significantly in comparison with toxigenic cultures. Fluorescent images showed fungal hyphae in dual culture displayed green fluorescent, and contacts of live hyphae were seen. A coconut agar plate assay was used to show that toxigenic A. flavus colony produced blue fluorescence under long UV exposure, suggesting that aflatoxin is exported outside fungal hyphae. Furthermore, the assay was applied to demonstrate the potential role of thigmo-regulation in fungal interaction. |
| تدمد: | 1867-1632 0178-7888 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::430948975e115adebd993596f18cda38 https://doi.org/10.1007/s12550-019-00364-w |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....430948975e115adebd993596f18cda38 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 18671632 01787888 |
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