Two-step fitness selection for intra-host variations in SARS-CoV-2
| العنوان: | Two-step fitness selection for intra-host variations in SARS-CoV-2 |
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| المؤلفون: | Jiarui Li, Pengcheng Du, Lijiang Yang, Ju Zhang, Chuan Song, Danying Chen, Yangzi Song, Nan Ding, Mingxi Hua, Kai Han, Rui Song, Wen Xie, Zhihai Chen, Xianbo Wang, Jingyuan Liu, Yanli Xu, Guiju Gao, Qi Wang, Lin Pu, Lin Di, Jie Li, Jinglin Yue, Junyan Han, Xuesen Zhao, Yonghong Yan, Fengting Yu, Angela R. Wu, Fujie Zhang, Yi Qin Gao, Yanyi Huang, Jianbin Wang, Hui Zeng, Chen Chen |
| المصدر: | Cell Reports |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, Adolescent, Genome, Viral, Polymorphism, Single Nucleotide, General Biochemistry, Genetics and Molecular Biology, Article, Young Adult, positive selection, Vaccine Development, Humans, Child, Phylogeny, intra-host variation, Aged, SARS-CoV-2, Infant, Newborn, COVID-19, Infant, Middle Aged, dynamic genetic divergences, Child, Preschool, Host-Pathogen Interactions, Mutation, Spike Glycoprotein, Coronavirus, Female |
| الوصف: | Spontaneous mutations introduce uncertainty into COVID-19 control procedures and vaccine development. Here, we perform spatio-temporal analysis on intra-host single-nucleotide variations (iSNVs) in 402 clinical samples from 170 patients, which reveals an increase in genetic diversity over time post-symptoms onset within individual patients. Nonsynonymous mutations are over-represented in the pool of iSNVs, but underrepresent at the single nucleotide polymorphism (SNP) level, suggesting a two-step fitness selection process: a large number of nonsynonymous substitutions are generated within the host (positive selection), and these substitutions tend to be unfixed as SNPs in population (negative selection). Dynamic iSNVs changes in subpopulations of different gender, age, illness severity and viral shedding time displayed a varied fitness selection process among populations. Taken together, our study highlights iSNVs provide a mutational pool shaping the virus rapid global evolution. Graphical Abstract The Intra-host variations in SARS-CoV-2 provide a mutational pool shaping the virus rapid global evolution. Here, Li et al. illustrate dynamic changes of iSNVs in a longitudinal cohort, and explored a two-step fitness selection process of within-host viral evolution. |
| تدمد: | 2211-1247 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4321555b6c52dcf6c42ec98bd87d00ef https://pubmed.ncbi.nlm.nih.gov/34982968 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4321555b6c52dcf6c42ec98bd87d00ef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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