The balance between MMP-2/-9 and TIMP-1/-2 is shifted towards MMP in renal cell carcinomas and can be further disturbed by hydrogen peroxide
| العنوان: | The balance between MMP-2/-9 and TIMP-1/-2 is shifted towards MMP in renal cell carcinomas and can be further disturbed by hydrogen peroxide |
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| المؤلفون: | Markus Heuser, Paul Thelen, Ulrike Johanns, Tobias Böttcher, Bernhard Hemmerlein, Julia Halbfass, Heinz-Joachim Radzun |
| المصدر: | International Journal of Oncology. |
| بيانات النشر: | Spandidos Publications, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Cancer Research, Cell, Matrix metalloproteinase, Kidney, medicine.disease_cause, Immunoenzyme Techniques, chemistry.chemical_compound, 0302 clinical medicine, Phorbol Esters, Tumor Cells, Cultured, RNA, Neoplasm, Hydrogen peroxide, chemistry.chemical_classification, 0303 health sciences, Cell cycle, Oxidants, Kidney Neoplasms, medicine.anatomical_structure, Matrix Metalloproteinase 9, Oncology, 030220 oncology & carcinogenesis, Matrix Metalloproteinase 2, Oxidation-Reduction, medicine.medical_specialty, Antigens, Differentiation, Myelomonocytic, Biology, 03 medical and health sciences, Antigens, CD, Internal medicine, medicine, Humans, Zymography, RNA, Messenger, Carcinoma, Renal Cell, 030304 developmental biology, Tissue Inhibitor of Metalloproteinase-2, Reactive oxygen species, Tissue Inhibitor of Metalloproteinase-1, Rhodamines, Macrophages, Hydrogen Peroxide, Molecular biology, Carcinoma, Papillary, Endocrinology, chemistry, Apoptosis, Case-Control Studies, Reactive Oxygen Species, Oxidative stress, Adenocarcinoma, Clear Cell |
| الوصف: | In malignant tumors the balance of matrix metalloproteinases (MMP) and tissue inhibitors of matrix metalloproteinases (TIMP) is disturbed. Radical oxygen species (ROS) and hydrogen peroxide potentially influence this balance. Therefore, we analyzed the balance of MMP and TIMP in renal cell carcinoma (RCC) specimens and cell lines. In RCC specimens MMP-2, MMP-9, TIMP-1, and TIMP-2 were immunohistochemically detected. Tumor-associated macrophages (TAM) as a potential source of ROS were characterized with an anti-CD68 antibody. Three RCC cell lines were treated with sublethal concentrations of hydrogen peroxide to simulate the effects of radical oxygen species. MMP-2 and MMP-9 protein expression was measured by zymography. mRNA expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 was assessed by quantitative reverse transcriptase polymerase chain reaction. Tumor cell-derived reactive oxygen species were measured by FACS analysis and dihydrorhodamine 123 oxidation. In RCCs the MMP and TIMP expression profile was variable. The balance between MMP and TIMP was shifted towards MMP in comparison to matched normal controls. TAM were localized in a close vicinity to MMP expresssing tumor cells. As in RCC specimens, the expression of MMP and TIMP in the analyzed RCC cell lines varied. Hydrogen peroxide induced MMP-2 and -9 mRNA and protein expression, whereas TIMP- 1 and TIMP-2 mRNA levels remained unaffected in cell lines. Thus, the ratio between MMP and TIMP was shifted towards MMP. Tumor cells did not increase the production of reactive oxygen species stimulation with phorbol ester or hydrogen peroxide. In RCC the balance between MMP and TIMP is disturbed. Oxidative stress potentially increases this imbalance. TAM might be one source of hydrogen peroxide thus supporting the invasive properties of RCCs. |
| تدمد: | 1791-2423 1019-6439 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43238f3eda7612d55f38b482809223d2 https://doi.org/10.3892/ijo.24.5.1069 |
| رقم الأكسشن: | edsair.doi.dedup.....43238f3eda7612d55f38b482809223d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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