Decreased expression of hypoxia-inducible factor 1α (HIF-1α) in cord blood monocytes under anoxia
| العنوان: | Decreased expression of hypoxia-inducible factor 1α (HIF-1α) in cord blood monocytes under anoxia |
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| المؤلفون: | Christiane Schlegel, Kai Liu, Bärbel Spring, Stefanie Dietz, Christian F. Poets, Hannes Hudalla, Trim Lajqi, Natascha Köstlin-Gille, Christian Gille |
| المصدر: | Pediatric Research. 93:870-877 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pediatrics, Perinatology and Child Health |
| الوصف: | Background Infections are a major cause for morbidity and mortality in neonates; however, the underling mechanisms for increased infection susceptibility are incompletely understood. Hypoxia, which is present in inflamed tissues, has been identified as an important activation signal for innate immune cells in adults and is mainly mediated by hypoxia-inducible factor 1α (HIF-1α). Fetal tissue pO2 physiologically is low but rises immediately after birth. Methods In this study, the effect of low oxygen partial pressure (pO2) on HIF-1α expression and its targets phagocytosis, reactive oxygen species (ROS) production and vascular endothelial growth factor (VEGF) secretion was compared in vitro between immune cells from adult peripheral blood and cord blood using anoxia, HIF-1α stabilizer desferroxamin (DFO) and E. coli as stimuli. Results We show that anoxia-induced HIF-1α protein accumulation, phagocytosis, ROS-production and VEGF-expression were greatly diminished in cord blood compared to adult cells. E. coli led to HIF-1α gene expression in adult and cord blood immune cells; however, cord blood cells failed to accumulate HIF-1α protein and VEGF upon E. coli stimulation. Conclusions Taken together, our results show a diminished activation of cord blood immune cells by low pO2, which might contribute to impaired reactivity in the context of infection. Impact Neonatal immune cells do not accumulate HIF-1α under low oxygen partial pressure leading to decreased phagocytosis and decreased ROS production. We demonstrate a previously unknown mechanism of reduced activation of neonatal immune cells in the context of an inflammatory response. This could contribute to the increased susceptibility of newborns and preterm infants to infection. |
| تدمد: | 1530-0447 0031-3998 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::435830501e813f8ae80e36b9fe31071c https://doi.org/10.1038/s41390-022-02193-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....435830501e813f8ae80e36b9fe31071c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15300447 00313998 |
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