A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing
| العنوان: | A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing |
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| المؤلفون: | Jonathan Y. Hsu, Martin J. Aryee, Ronghao Zhou, J. Keith Joung, Lukas M. Langner, Sara P. Garcia, Bret R. Miller, Julian Grünewald, Sowmya Iyer, Caleb A. Lareau |
| المصدر: | Nature biotechnology |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0303 health sciences, Single type, Biomedical Engineering, RNA, Bioengineering, Computational biology, Base (topology), Applied Microbiology and Biotechnology, Article, DNA sequencing, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, chemistry, Genome editing, Molecular Medicine, CRISPR, 030217 neurology & neurosurgery, Cytosine, DNA, 030304 developmental biology, Biotechnology |
| الوصف: | Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors. Editorial summary Base editor that concurrently modifies both adenine and cytosine broadens the potential applications of base editing. |
| تدمد: | 1546-1696 1087-0156 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4372198978a9c533af57b06ded868f53 https://doi.org/10.1038/s41587-020-0535-y |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4372198978a9c533af57b06ded868f53 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15461696 10870156 |
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