Cytokine and hormonal regulation of bone marrow immune cell Wnt10b expression
| العنوان: | Cytokine and hormonal regulation of bone marrow immune cell Wnt10b expression |
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| المؤلفون: | Fraser L. Collins, Naiomy Deliz Rios-Arce, Narayanan Parameswaran, Laura R. McCabe |
| المصدر: | PLoS ONE, Vol 12, Iss 8, p e0181979 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_treatment, Gene Expression, Parathyroid hormone, lcsh:Medicine, Cell Count, White Blood Cells, Spectrum Analysis Techniques, Animal Cells, Medicine and Health Sciences, Cytotoxic T cell, lcsh:Science, Mice, Knockout, Mice, Inbred BALB C, Multidisciplinary, T Cells, Flow Cytometry, medicine.anatomical_structure, Cytokine, Spectrophotometry, Female, Tumor necrosis factor alpha, Cytophotometry, Cellular Types, Research Article, medicine.medical_specialty, medicine.drug_class, Immune Cells, Immunology, Bone Marrow Cells, Cytotoxic T cells, Biology, Research and Analysis Methods, 03 medical and health sciences, Immune system, Internal medicine, Genetics, medicine, Animals, Blood Cells, Tumor Necrosis Factor-alpha, Macrophages, lcsh:R, Biology and Life Sciences, Estrogens, Cell Biology, Mice, Inbred C57BL, Wnt Proteins, 030104 developmental biology, Endocrinology, Estrogen, lcsh:Q, Bone marrow, CD8, Granulocytes |
| الوصف: | Background & aims Wnt10b is a crucial regulator of bone density through its ability to promote osteoblastogenesis. Parathyroid hormone has been shown to regulate Wnt10b expression in CD8+ T cells. However, the relative expression and other source(s) of Wnt10b in the bone marrow immune cells (BMICs) is unknown. Sex hormones and cytokines such as, estrogen and TNFα are critical regulators of bone physiology but whether they regulate BMIC Wnt10b expression is unclear. To determine the potential regulation of Wnt10b by estrogen and TNFα, we assessed Wnt10b expression by flow cytometry under estrogen- and TNFα-deficient conditions. Methods Effects of TNFα was determined in male and female C57BL/6 wildtype and TNFα knockout mice. Effect of estrogen was investigated 4, 6 and 8 weeks post-surgery in ovariectomized Balb/c mice. Intracellular Wnt10b was detected using goat anti-mouse Wnt10b and a conjugated secondary antibody and analyzed by flow cytometry. Results Wnt10b expression was sex- and lineage-specific. Females had 1.8-fold higher Wnt10b signal compared to males. Percent of Wnt10b+ myeloid cells was higher in females than males (8.9% Vs 5.4%) but Wnt10b+ lymphoid cells was higher in males than females (6.3% Vs 2.5%). TNFα ablation in males increased total BM Wnt10b expression 1.5-fold but significantly reduced the percentage of BM Wnt10b+ CD4+ T cells (65%), CD8+ T cells (59%), dendritic cells (59%), macrophages (56%) and granulocytes (52%). These effects of TNFα on Wnt10b were observed only in males. In contrast to TNFα, estrogen-deficiency had indirect effects on BMIC Wnt10b levels; reducing the average percentage of BM Wnt10b+ CD8+ T cells (25%) and granulocytes (26%) across an 8-week time course. Conclusion Our results demonstrate unique cell type- and sex-dependent effects on BMIC Wnt10b expression. Together, our results reveal myeloid cells in the bone marrow as an important source of Wnt10b under complex hormonal and cytokine regulation. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43aa63203b59a4b2d3fa72bbb7ab34f3 http://europepmc.org/articles/PMC5553813?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....43aa63203b59a4b2d3fa72bbb7ab34f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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