Experimental and Computational Investigation of Biofilm Formation by Rhodopseudomonas palustris Growth under Two Metabolic Modes
| العنوان: | Experimental and Computational Investigation of Biofilm Formation by Rhodopseudomonas palustris Growth under Two Metabolic Modes |
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| المؤلفون: | Rebecca Christianson, Chase Kernan, Philicia P. Chow, Jean J. Huang |
| المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 6, p e0129354 (2015) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | food.ingredient, Light, Science, Confocal, Phototrophic biofilms, Biology, 03 medical and health sciences, food, Confocal imaging, Botany, Hypoxia, 030304 developmental biology, 0303 health sciences, Multidisciplinary, Microscopy, Confocal, Phototroph, 030306 microbiology, Biofilm, Rhodopseudomonas, biochemical phenomena, metabolism, and nutrition, Models, Theoretical, biology.organism_classification, Biofilms, Biophysics, Medicine, Rhodopseudomonas palustris, Bacteria, Research Article |
| الوصف: | We examined biofilms formed by the metabolically versatile bacterium Rhodopseudomonas palustris grown via different metabolic modes. R. palustris was grown in flow cell chambers with identical medium conditions either in the presence or absence of light and oxygen. In the absence of oxygen and the presence of light, R. palustris grew and formed biofilms photoheterotrophically, and in the presence of oxygen and the absence of light, R. palustris grew and formed biofilms heterotrophically. We used confocal laser scanning microscopy and image analysis software to quantitatively analyze and compare R. palustris biofilm formation over time in these two metabolic modes. We describe quantifiable differences in structure between the biofilms formed by the bacterium grown heterotrophically and those grown photoheterotrophically. We developed a computational model to explore ways in which biotic and abiotic parameters could drive the observed biofilm architectures, as well as a random-forest machine-learning algorithm based on structural differences that was able to identify growth conditions from the confocal imaging of the biofilms with 87% accuracy. Insight into the structure of phototrophic biofilms and conditions that influence biofilm formation is relevant for understanding the generation of biofilm structures with different properties, and for optimizing applications with phototrophic bacteria growing in the biofilm state. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43d821d84dd1f859b5befd273ac34f32 https://pubmed.ncbi.nlm.nih.gov/26087200 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....43d821d84dd1f859b5befd273ac34f32 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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