The genetic basis of phage susceptibility, cross-resistance and host-range in Salmonella
| العنوان: | The genetic basis of phage susceptibility, cross-resistance and host-range in Salmonella |
|---|---|
| المؤلفون: | Benjamin A. Adler, Alexey E. Kazakov, Crystal Zhong, Hualan Liu, Elizabeth Kutter, Lauren M. Lui, Torben N. Nielsen, Heloise Carion, Adam M. Deutschbauer, Vivek K. Mutalik, Adam P. Arkin |
| المصدر: | Microbiology (Reading, England), vol 167, iss 12 Microbiology |
| بيانات النشر: | eScholarship, University of California, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Salmonella typhimurium, viruses, salmonella, Microbiology, Host Specificity, genome-wide, resistance, Vaccine Related, host-range, wide, phage, Genetics, 2.1 Biological and endogenous factors, Bacteriophages, Aetiology, genome, Antimicrobials and AMR, Virulence, Human Genome, Foodborne Illness, Emerging Infectious Diseases, Infectious Diseases, host, genetic screen, range, sigma factor, Salmonella Phages, Infection |
| الوصف: | Though bacteriophages (phages) are known to play a crucial role in bacterial fitness and virulence, our knowledge about the genetic basis of their interaction, cross-resistance and host-range is sparse. Here, we employed genome-wide screens in Salmonella enterica serovar Typhimurium to discover host determinants involved in resistance to eleven diverse lytic phages including four new phages isolated from a therapeutic phage cocktail. We uncovered 301 diverse host factors essential in phage infection, many of which are shared between multiple phages demonstrating potential cross-resistance mechanisms. We validate many of these novel findings and uncover the intricate interplay between RpoS, the virulence-associated general stress response sigma factor and RpoN, the nitrogen starvation sigma factor in phage cross-resistance. Finally, the infectivity pattern of eleven phages across a panel of 23 genome sequenced Salmonella strains indicates that additional constraints and interactions beyond the host factors uncovered here define the phage host range. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::440dfd78b0569af6fb0259b778eba5b2 https://escholarship.org/uc/item/6z4571b3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....440dfd78b0569af6fb0259b778eba5b2 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |