Biological-Profiling-Based Systematic Analysis of Rhizoma Coptidis from Different Growing Regions and Its Anticholesterol Biosynthesis Activity on HepG2 Cells
| العنوان: | Biological-Profiling-Based Systematic Analysis of Rhizoma Coptidis from Different Growing Regions and Its Anticholesterol Biosynthesis Activity on HepG2 Cells |
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| المؤلفون: | Lan Xie, Kun Luo, Haoxun Li, Juan Feng, Weifang Song, Xing Wanli, Wenlong Zhao, Guo Hongyan, Jing Cheng, Ruijun Wang, Dang Honglei, Sun Yimin |
| المصدر: | Molecular Pharmaceutics. 15:2234-2245 |
| بيانات النشر: | American Chemical Society (ACS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Coptis chinensis, food.ingredient, Berberine, Sterol response element binding, Chemistry, Alkaloid, Pharmaceutical Science, Hyperlipidemias, Hep G2 Cells, In vitro, Biological pathway, chemistry.chemical_compound, Cholesterol, food, Biochemistry, Biosynthesis, Herb, Drug Discovery, Humans, Molecular Medicine, Mevalonate pathway, Drugs, Chinese Herbal, Hypolipidemic Agents, Sterol Regulatory Element Binding Protein 2 |
| الوصف: | Rhizoma Coptidis is a widely cultivated traditional Chinese herb. Although the chemical profiles of Rhizoma Coptidis have been established previously, the biological profiling of Rhizoma Coptidis has not been conducted yet. In this study, we collected Rhizoma Coptidis varieties from four distinct growing regions and performed genome-wide biological response fingerprinting (BioReF) on HepG2 cells using a gene expression array. Similar biological pathways were affected by extracts of all four Rhizoma Coptidis varieties but not by their analogue, Mahoniae Caulis. Among these pathways, the terpenoid backbone biosynthesis pathway was highly enriched, and six genes in the mevalonate (MVA) pathway were all down-regulated. However, the expression, maturation, as well as the specific DNA binding capacity of their coordinate transcription factor, sterol response element binding protein 2 (SREBP2), was not affected by Rhizoma Coptidis extract (RCE) or its typical active alkaloid berberine. Cellular cholesterol content tests further verified the cholesterol-lowering function of RCE in vitro, which supplements evidence for the use of Rhizoma Coptidis in hyperlipidemia treatment. This is the first described example of evaluating the quality of Rhizoma Coptidis with BioReF and a good demonstration of using BioReF to uncover the mechanisms of herbs at a systematic level. |
| تدمد: | 1543-8392 1543-8384 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b6ac155c22ee1f5dd61402e224be59 https://doi.org/10.1021/acs.molpharmaceut.8b00078 |
| رقم الأكسشن: | edsair.doi.dedup.....44b6ac155c22ee1f5dd61402e224be59 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15438392 15438384 |
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