The transcription factors hypoxia-inducible factor 1? and Ets-1 colocalize in the hypoxic synovium of inflamed joints in adjuvant-induced arthritis
| العنوان: | The transcription factors hypoxia-inducible factor 1? and Ets-1 colocalize in the hypoxic synovium of inflamed joints in adjuvant-induced arthritis |
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| المؤلفون: | David R. Blake, Paul I. Mapp, Claire E. Lewis, Christopher L. Peters, V R Winrow, C. J. Morris |
| المصدر: | Arthritis & Rheumatism. 50:291-296 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | musculoskeletal diseases, medicine.medical_specialty, Pathology, Immunology, Arthritis, Inflammation, Proto-Oncogene Protein c-ets-1, Rheumatology, Proto-Oncogene Proteins, Internal medicine, medicine, Animals, Immunology and Allergy, Pharmacology (medical), Hypoxia, Synovitis, Proto-Oncogene Proteins c-ets, business.industry, Synovial Membrane, Colocalization, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Arthritis, Experimental, Immunohistochemistry, Rats, Disease Models, Animal, Rats, Inbred Lew, Rheumatoid arthritis, Experimental pathology, Female, medicine.symptom, business, Transcription Factors |
| الوصف: | Objective To determine the relationship between hypoxia and the expression of Ets-1 and hypoxia-inducible factor 1α (HIF-1α) in both normal and inflamed joints. Adjuvant-induced arthritis (AIA) was used as the model system, since it mirrors many aspects of the pathology of rheumatoid arthritis. Methods Adjuvant arthritis was induced in a group of 10 female Lewis rats. A second group of 10 uninjected female Lewis rats served as naive controls. When a maximum clinical joint score was achieved in the AIA group, all 20 rats were injected with the specific hypoxic cell marker Hypoxyprobe-1 and subsequently killed. Hypoxyprobe-1 adducts, Ets-1, and HIF-1α were localized in the joints of the hind feet from these groups using immunohistochemistry. Results Compared with the joints from control rats, inflamed joints contained markedly more cells with Hypoxyprobe-1 adduct immunoreactivity, Ets-1–immunoreactive nuclei, and nuclear immunoreactivity for both Ets-1 and HIF-1α. Conclusion Our results demonstrate the presence of hypoxia in inflamed joints in this experimental model of arthritis. The colocalization of Ets-1 and HIF-1α in these hypoxic areas suggests that hypoxia may induce Ets-1 and HIF-1α expression during joint inflammation. |
| تدمد: | 1529-0131 0004-3591 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44ce9e2cccf2bdc448dccce6dbd050ac https://doi.org/10.1002/art.11473 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....44ce9e2cccf2bdc448dccce6dbd050ac |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15290131 00043591 |
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