Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells
| العنوان: | Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells |
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| المؤلفون: | Ah Ra Goh, Yong-Soo Bae, Hyung-Joo Kwon, Soo Young Choi, Sung Mi Ju, Dong-Joo Kwon, Jinseu Park, Gi Soo Youn |
| المصدر: | Molecules and Cells. 33:335-341 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | MAPK/ERK pathway, beta-Defensins, MAP Kinase Kinase 4, Antimicrobial peptides, MAP Kinase Kinase 1, Biology, Transfection, Cell Line, Humans, Molecular Biology, B-Lymphocytes, Messenger RNA, Reporter gene, Beta-defensin 2, NF-kappa B, Articles, Cell Biology, General Medicine, Molecular biology, Transcription Factor AP-1, Beta defensin, Gene Expression Regulation, Cell culture, Dactinomycin, HIV-1, tat Gene Products, Human Immunodeficiency Virus |
| الوصف: | Defensins, a family of antimicrobial peptides, are one of the first lines of host defense. Human beta-defensins (hBD) such as hBD-2 and -3 have anti-HIV activity. Previous studies have shown that HIV-1 virion can induce the expression of hBD, although the exact components of HIV-1 virion that are responsible for hBD expression have not yet been elucidated. In this study, we examined the effect of HIV-1 Tat on the expression of hBD in B cells. Stimulation of B cells with HIV-1 Tat protein significantly increased the mRNA and protein levels of hBD-2. HIV-1 Tat also induced the activation of a reporter gene for hBD-2 in a dose-dependent manner in B cells. Pretreatment of B cells with a JNK inhibitor suppressed HIV-1 Tat-induced hBD-2 expression. Pretreatment of B cells with AP-1 inhibitors or NF-κB inhibitors led to a decrease in HIV-1 Tat-induced protein and mRNA expression of hBD-2. Taken together, our results indicate that HIV-1 Tat can up-regulate the expression of hBD-2 via JNK-NF-κB/AP-1-dependent pathways in human B cells. |
| تدمد: | 0219-1032 1016-8478 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::450539543383317d152c104828b2cbe1 https://doi.org/10.1007/s10059-012-2287-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....450539543383317d152c104828b2cbe1 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 02191032 10168478 |
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