HuR expression is a marker of poor prognosis in renal cell carcinoma
| العنوان: | HuR expression is a marker of poor prognosis in renal cell carcinoma |
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| المؤلفون: | Hanna Ronkainen, Pasi Hirvikoski, Markku H. Vaarala, Ari Ristimäki |
| المصدر: | Tumor Biology. 32:481-487 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult, Male, Pathology, medicine.medical_specialty, Small interfering RNA, medicine.disease_cause, ELAV-Like Protein 1, Renal cell carcinoma, Cell Line, Tumor, Biomarkers, Tumor, medicine, Carcinoma, Humans, Carcinoma, Renal Cell, Aged, Aged, 80 and over, Kidney, business.industry, RNA-Binding Proteins, Cancer, General Medicine, Middle Aged, Prognosis, medicine.disease, Kidney Neoplasms, medicine.anatomical_structure, ELAV Proteins, Cyclooxygenase 2, Antigens, Surface, Cancer research, Immunohistochemistry, Female, business, Carcinogenesis, Immunostaining |
| الوصف: | The HuR protein is a nucleocytoplasmic protein which plays an important role in the regulation of mRNA stability, and dysregulation of its expression has been linked to carcinogenesis. We studied 152 patients with primary renal cell carcinoma (RCC) who underwent surgery for the removal of kidney tumours between 1990 and 1999. The mean follow-up was 90 months. The expression of HuR and cyclooxygenase-2 (COX-2) was determined by immunohistochemistry using monoclonal antibodies. The immunostaining results were associated with patient age, clinical stage, Fuhrman grade and patient outcome. Cytoplasmic expression of HuR and COX-2 was positive in 37 (25%) and 22 (15%) of the tumours, respectively. The expression of HuR was associated with stage. The expression of COX-2 was associated with stage and nuclear grade. The RCC-specific survival was reduced in patients whose tumours expressed HuR or COX-2. The hazard ratio (HR) of patients with HuR-expressing tumours was 2.18 (95% confidence interval (CI), 1.16-4.09; p = 0.015) and the HR of patients with COX-2-expressing tumours was 2.29 (95% CI, 1.15-4.54; p = 0.018). In the Cox regression analysis the only independent prognostic factor was stage (p < 0.001). Treatment of an RCC cell line (769-P) with HuR-targeted small interfering RNA resulted in the reduced expression of HuR and COX-2. We conclude that cytoplasmic HuR expression is associated with reduced RCC-specific survival. The HuR protein regulates the expression of COX-2 in RCC cells, which is one potential mechanism of action for the HuR-associated aggressive behaviour of RCC. |
| تدمد: | 1423-0380 1010-4283 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::450e91bd6d993092773bcfae584ddc32 https://doi.org/10.1007/s13277-010-0141-6 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....450e91bd6d993092773bcfae584ddc32 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14230380 10104283 |
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