Structure determination of the O-antigenic polysaccharide from the enteroinvasive Escherichia coli O136
| العنوان: | Structure determination of the O-antigenic polysaccharide from the enteroinvasive Escherichia coli O136 |
|---|---|
| المؤلفون: | Göran Widmalm, Andrej Weintraub, Mikael Staaf |
| المصدر: | European Journal of Biochemistry. 263:656-661 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Carbon Isotopes, Magnetic Resonance Spectroscopy, Anomer, Geminal, Stereochemistry, Chemistry, Chemical shift, Molecular Sequence Data, Absolute configuration, O Antigens, Nuclear Overhauser effect, medicine.disease_cause, Methylation, Biochemistry, Carbohydrate Sequence, Heteronuclear molecule, Carbohydrate Conformation, Escherichia coli, medicine, Two-dimensional nuclear magnetic resonance spectroscopy, Enteroinvasive Escherichia coli, Hydrogen |
| الوصف: | The structure of the O-antigen polysaccharide of the lipopolysaccharide from the enteroinvasive Escherichia coli O136 has been elucidated. The composition of the repeating unit was established by sugar and methylation analysis together with 1H and 13C NMR spectroscopy. Two-dimensional nuclear Overhauser effect spectroscopy (NOESY) and heteronuclear multiple-bond correlation experiments were used to deduce the sequence. The absolute configuration for the nonulosonic acid (NonA) could be determined using spin-spin coupling constants, 13C chemical shifts and NOESY. The anomeric configuration of the NonA was determined via vicinal and geminal 13C,1H coupling constants. The structure of the repeating unit of the polysaccharide from E. coli O136 is as follows, in which beta-NonpA is 5,7-diacetamido-3,5,7, 9-tetradeoxy-Lglycero-beta-Lmanno-nonulosonic acid: -->4)-beta-NonpA-(2-->4)-beta-D-Galp-(1-->4)-beta-D-GlcpNAc-(1--> |
| تدمد: | 1432-1033 0014-2956 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::452513d70de1a32dc0e8b5d8500ad7e1 https://doi.org/10.1046/j.1432-1327.1999.00531.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....452513d70de1a32dc0e8b5d8500ad7e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
|---|